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  • © 2013

Immobilization of Enzymes and Cells

Editors:

  • Demonstrates simple and efficient protocols for the preparation, characterization, and utilization of immobilized enzymes and cells
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 1051)

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Table of contents (24 protocols)

  1. Front Matter

    Pages i-xi
  2. Immobilization of Enzymes: A Literature Survey

    • Beatriz Brena, Paula González-Pombo, Francisco Batista-Viera
    Pages 15-31
  3. Glutaraldehyde-Mediated Protein Immobilization

    • Fernando López-Gallego, Jose M. Guisán, Lorena Betancor
    Pages 33-41
  4. Stabilization of Enzymes by Multipoint Covalent Immobilization on Supports Activated with Glyoxyl Groups

    • Fernando López-Gallego, Gloria Fernandez-Lorente, Javier Rocha-Martin, Juan M. Bolivar, Cesar Mateo, Jose M. Guisan
    Pages 59-71
  5. Oriented Covalent Immobilization of Enzymes on Heterofunctional-Glyoxyl Supports

    • Cesar Mateo, Gloria Fernandez-Lorente, Javier Rocha-Martin, Juan M. Bolivar, Jose M. Guisan
    Pages 73-88
  6. Reversible Covalent Immobilization of Enzymes via Disulfide Bonds

    • Karen Ovsejevi, Carmen Manta, Francisco Batista-Viera
    Pages 89-116
  7. Immobilization of Enzymes by Bioaffinity Layering

    • Veena Singh, Meryam Sardar, Munishwar Nath Gupta
    Pages 129-137
  8. Immobilization of Enzymes on Magnetic Beads Through Affinity Interactions

    • Audrey Sassolas, Akhtar Hayat, Jean-Louis Marty
    Pages 139-148
  9. Tips for the Functionalization of Nanoparticles with Antibodies

    • Ester Polo, Sara Puertas, María Moros, Pilar Batalla, José M. Guisán, Jesús M. de la Fuente et al.
    Pages 149-163
  10. Design and Characterization of Functional Nanoparticles for Enhanced Bio-performance

    • Pablo del Pino, Scott G. Mitchell, Beatriz Pelaz
    Pages 165-207
  11. Immobilization of Enzymes on Ethynyl-Modified Electrodes via Click Chemistry

    • Akhtar Hayat, Audrey Sassolas, Amina Rhouati, Jean-Louis Marty
    Pages 209-216
  12. Modification of Carbon Nanotube Electrodes with 1-Pyrenebutanoic Acid, Succinimidyl Ester for Enhanced Bioelectrocatalysis

    • Guinevere Strack, Robert Nichols, Plamen Atanassov, Heather R. Luckarift, Glenn R. Johnson
    Pages 217-228
  13. Enzyme Immobilization by Entrapment Within a Gel Network

    • Audrey Sassolas, Akhtar Hayat, Jean-Louis Marty
    Pages 229-239
  14. Improving Lipase Activity by Immobilization and Post-immobilization Strategies

    • Jose M. Palomo, Marco Filice, Oscar Romero, Jose M. Guisan
    Pages 255-273
  15. Biomedical Applications of Immobilized Enzymes: An Update

    • Marta Pastor, Amaia Esquisabel, José Luis Pedraz
    Pages 285-299

About this book

For most of industrial applications, enzymes and cells have to be immobilized, via very simple and cost-effective protocols,  in order to be re-used for very long periods of time. From this point of view, immobilization, simplicity and stabilization have to be strongly related concepts. The third edition of Immobilization of Enzymes and Cells expands upon and updates the previous editions with current, detailed protocols for immobilization. With new chapters on protocols for immobilization of enzymes and cells which may be useful to greatly improve the functional properties of enzymes and cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

 

Authoritative and practical, Immobilization of Enzymes and Cells, Third Edition demonstrates simple and efficient protocols for the preparation, characterization, and utilization of immobilized enzymes and cells.

Editors and Affiliations

  • Instituto de Catlisis y Petroleoquimica, CSIC, Madrid, Spain

    Jose M. Guisan

Bibliographic Information

  • Book Title: Immobilization of Enzymes and Cells

  • Editors: Jose M. Guisan

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-62703-550-7

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, New York 2013

  • Hardcover ISBN: 978-1-62703-549-1Published: 11 August 2013

  • Softcover ISBN: 978-1-4939-5988-4Published: 27 August 2016

  • eBook ISBN: 978-1-62703-550-7Published: 13 August 2013

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 3

  • Number of Pages: XI, 377

  • Number of Illustrations: 77 b/w illustrations, 39 illustrations in colour

  • Topics: Cell Biology, Enzymology

Buy it now

Buying options

eBook USD 89.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 119.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 169.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access