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Nucleic Acid Detection

Methods and Protocols

  • Book
  • © 2013

Overview

Editors:
  1. Dmitry M. Kolpashchikov

    1. University of Central Florida Department of Chemistry, Orlando, USA

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    You can also search for this editor in PubMed   Google Scholar

  2. Yulia V. Gerasimova

    1. University of Central Florida Department of Chemistry, Orlando, USA

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 1039)

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Table of contents (24 protocols)

  1. Front Matter

    Pages i-xiv
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  3. New Real-Time and Instantaneous Assays

    1. Front Matter

      Pages 29-29
      Download chapter PDF

    2. MNAzyme qPCR: A Superior Tool for Multiplex qPCR

      • Elisa Mokany, Alison V. Todd
      Pages 31-49

    3. A New, Multiplex, Quantitative Real-Time Polymerase Chain Reaction System for Nucleic Acid Detection and Quantification

      • Fang Liang, Neetika Arora, Kang Liang Zhang, David Che Cheng Yeh, Richard Lai, Darnley Pearson et al.
      Pages 51-68

    4. Detection of SNP-Containing Human DNA Sequences Using a Split Sensor with a Universal Molecular Beacon Reporter

      • Yulia V. Gerasimova, Jack Ballantyne, Dmitry M. Kolpashchikov
      Pages 69-80

    5. SNP Analysis Using a Molecular Beacon-Based Operating Cooperatively (OC) Sensor

      • Evan M. Cornett, Dmitry M. Kolpashchikov
      Pages 81-86

  4. Isothermal Amplification Methods for Nucleic Acid Detection

    1. Front Matter

      Pages 87-87
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    2. Detection of rpoB Gene Mutations Using Helicase-Dependent Amplification

      • Wanyuan Ao, Robert Jenison
      Pages 89-98

    3. Rapid Detection of Brucella spp. Using Loop-Mediated Isothermal Amplification (LAMP)

      • Shouyi Chen, Xunde Li, Juntao Li, Edward R. Atwill
      Pages 99-108

    4. Loop-Mediated Isothermal Amplification Method for a Differential Identification of Human Taenia Tapeworms

      • Yasuhito Sako, Agathe Nkouawa, Tetsuya Yanagida, Akira Ito
      Pages 109-120

    5. Detection of Mutation by Allele-Specific Loop-Mediated Isothermal Amplification (AS-LAMP)

      • Hiroka Aonuma, Athanase Badolo, Kiyoshi Okado, Hirotaka Kanuka
      Pages 121-127

  5. Signal Amplification Approach

    1. Front Matter

      Pages 129-129
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    2. DNA Detection by Cascade Enzymatic Signal Amplification

      • Bingjie Zou, Yinjiao Ma, Guohua Zhou
      Pages 131-137

  6. Non-fluorescent Detection Formats

    1. Front Matter

      Pages 139-139
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    2. Visual DNA Detection and SNP Genotyping Using Asymmetric PCR and Split DNA Enzymes

      • Jia Ling Neo, Mahesh Uttamchandani
      Pages 141-151

    3. Detection of Single-Stranded Nucleic Acids via Colorimetric Means, Using G-Quadruplex Probes

      • Herman O. Sintim, Shizuka Nakayama
      Pages 153-159

    4. Lateral Flow Biosensors for the Detection of Nucleic Acid

      • Lingwen Zeng, Puchang Lie, Zhiyuan Fang, Zhuo Xiao
      Pages 161-167
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Keywords

About this book

In Nucleic Acid Chemistry: Methods and Protocols, expert researches in the field detail techniques and approaches for the detection of DNA and RNA. These techniques include the recovery of trace amounts of DNA for amplification and analysis, new qPCR chemistries, new application of isothermal amplification techniques, assays with visual or electric signals for point-of-care diagnostics, improvement of fluorescent in situ hybridization, and new signal amplification techniques. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

 

Authoritative and practical, Nucleic Acid Chemistry: Methods and Protocols seeks to aid scientists in the further study of detection for DNA and RNA.

Editors and Affiliations

  • University of Central Florida Department of Chemistry, Orlando, USA

    Dmitry M. Kolpashchikov, Yulia V. Gerasimova

Bibliographic Information

  • Book Title: Nucleic Acid Detection

  • Book Subtitle: Methods and Protocols

  • Editors: Dmitry M. Kolpashchikov, Yulia V. Gerasimova

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-62703-535-4

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, New York 2013

  • Hardcover ISBN: 978-1-62703-534-7Published: 13 September 2013

  • Softcover ISBN: 978-1-4939-6041-5Published: 23 August 2016

  • eBook ISBN: 978-1-62703-535-4Published: 12 September 2013

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XIV, 320

  • Number of Illustrations: 27 b/w illustrations, 58 illustrations in colour

  • Topics: Human Genetics, Nucleic Acid Chemistry

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