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Antiviral Methods and Protocols

  • Book
  • © 2013

Overview

Editors:
  1. Edwin Yunhao Gong

    1. Janssen Infectious Diseases-Diagnostics, Johnson & Johnson Corporation, Beerse, Belgium

    View editor publications

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  • Features all new techniques for antiviral research
  • Contains detailed protocols from labs around the world
  • Includes expert tips and key implementation advice to ensure successful results

Part of the book series: Methods in Molecular Biology (MIMB, volume 1030)

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Table of contents (33 protocols)

  1. Front Matter

    Pages i-xiv
    Download chapter PDF

  2. Human Immunodeficiency Virus Type 1 (HIV-1)

    1. Front Matter

      Pages 1-1
      Download chapter PDF

    2. A Fluorescence-Based High-Throughput Screening Assay to Identify HIV-1 Inhibitors

      • Peggy Geluykens, Koen Van Acker, Johan Vingerhoets, Christel Van den Eynde, Marnix Van Loock, Géry Dams
      Pages 3-9

    3. A Homogeneous Time-Resolved Fluorescence Assay to Identify Inhibitors of HIV-1 Fusion

      • Liesbet Smeulders, Lieve Bunkens, Inge Vereycken, Koen Van Acker, Pascale Holemans, Emmanuel Gustin et al.
      Pages 11-17

    4. Identification of HIV-1 Reverse Transcriptase Inhibitors Using a Scintillation Proximity Assay

      • Bertrand Van Schoubroeck, Marnix Van Loock, Tania Ivens, Pascale Dehertogh, Dirk Jochmans, Géry Dams
      Pages 19-24

    5. Biochemical Screening Assays to Identify HIV-1 Integrase Inhibitors

      • Marleen Clynhens, Alexandra Smets, Inge Vereycken, Marnix Van Loock, Reginald Clayton, Geert Meersseman et al.
      Pages 25-36

    6. HIV-1 Genotyping of the Protease-Reverse Transcriptase and Integrase Genes to Detect Mutations That Confer Antiretroviral Resistance

      • Peter Van den Eede, Liesbeth Van Wesenbeeck, Yvan Verlinden, Maxim Feyaerts, Veerle Smits, Ann Verheyen et al.
      Pages 37-55

  3. Hepatitis Viruses

    1. Front Matter

      Pages 57-57
      Download chapter PDF

    2. In Vitro Kinetic Profiling of Hepatitis C Virus NS3 Protease Inhibitors by Progress Curve Analysis

      • Rumin Zhang, William T. Windsor
      Pages 59-79

    3. A Novel Hepatitis C Virus NS5B Polymerase Assay of De Novo Initiated RNA Synthesis Directed from a Heteropolymeric RNA Template

      • Eric Ferrari, Hsueh-Cheng Huang
      Pages 81-92

    4. Selecting and Characterizing Drug-Resistant Hepatitis C Virus Replicon

      • Inge Vliegen, Leen Delang, Johan Neyts
      Pages 93-103

    5. A Cellular Replicon-Based Phenotyping Assay to Determine Susceptibility of Hepatitis C Virus Clinical Isolates to NS3/4A Protease Inhibitors

      • Leen Vijgen, Jannick Verbeeck, Barbara Van Kerckhove, Jan Martin Berke, Diana Koletzki, Gregory Fanning et al.
      Pages 105-117

    6. Expression and Purification of Hepatitis C Virus Protease from Clinical Samples

      • Liesbet van der Helm
      Pages 119-127

    7. Surface Plasmon Resonance as a Tool to Select Potent Drug Candidates for Hepatitis C Virus NS5B Polymerase

      • Erna Cleiren, Benoit Devogelaere, Katleen Fierens
      Pages 129-136

    8. Amplification and Sequencing of the Hepatitis C Virus NS3/4A Protease and the NS5B Polymerase Regions for Genotypic Resistance Detection of Clinical Isolates of Subtypes 1a and 1b

      • Diana Koletzki, Theresa Pattery, Bart Fevery, Leen Vanhooren, Lieven J. Stuyver
      Pages 137-149

    9. A Southern Blot Assay for Detection of Hepatitis B Virus Covalently Closed Circular DNA from Cell Cultures

      • Dawei Cai, Hui Nie, Ran Yan, Ju-Tao Guo, Timothy M. Block, Haitao Guo
      Pages 151-161

    10. In Vitro Phenotyping of Recombinant Hepatitis B Virus Containing the Polymerase/Reverse Transcriptase Gene from Clinical Isolates

      • Yang Liu, Kathryn M. Kitrinos
      Pages 163-181

  4. Flaviviruses

    1. Front Matter

      Pages 183-183
      Download chapter PDF

    2. Cell-Based Antiviral Assays for Screening and Profiling Inhibitors Against Dengue Virus

      • Edwin Yunhao Gong, Marleen Clynhens, Tania Ivens, Pedro Lory, Kenny Simmen, Guenter Kraus
      Pages 185-194

    3. A Duplex Real-Time RT-PCR Assay for Profiling Inhibitors of Four Dengue Serotypes

      • Edwin Yunhao Gong, Alexandra Smets, Nick Verheyen, Marleen Clynhens, Emmanuel Gustin, Pedro Lory et al.
      Pages 195-203
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Keywords

About this book

Although antiviral drugs have been successfully developed for some viral diseases, there remains a clear, unmet medical need to develop novel antiviral agents for the control and management of many viruses that currently have no or limited treatment options as well as a need to overcome the limitations associated with the existing antiviral drugs, such as adverse effects and emergence of drug-resistant mutations. The second edition of Antiviral Methods and Protocols features:

  • All chapters are new and written by experts in the field, reflecting the major recent technical advances in antiviral research and discovery.
  • This edition focuses on many important human viruses, such as human immunodeficiency virus type 1 (HIV-1), hepatitis viruses (hepatitis B and C viruses), herpes viruses, human respiratory syncytial virus (RSV), and influenza virus, while also featuring some important emerging viruses, such as dengue virus, West Nile virus, and chikungunya virus. 
  • As a volume in the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 

Comprehensive and cutting-edge, Antiviral Methods and Protocols, Second Edition will serve as an excellent laboratory reference for pharmaceutical and academic biologists, medicinal chemists, and pharmacologists as well as for virologists in the field of antiviral research and drug discovery.

Editors and Affiliations

  • Janssen Infectious Diseases-Diagnostics, Johnson & Johnson Corporation, Beerse, Belgium

    Edwin Yunhao Gong

Bibliographic Information

  • Book Title: Antiviral Methods and Protocols

  • Editors: Edwin Yunhao Gong

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-62703-484-5

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC 2013

  • Hardcover ISBN: 978-1-62703-483-8Published: 03 July 2013

  • Softcover ISBN: 978-1-4939-6035-4Published: 23 August 2016

  • eBook ISBN: 978-1-62703-484-5Published: 03 July 2013

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 2

  • Number of Pages: XIV, 452

  • Number of Illustrations: 24 b/w illustrations, 28 illustrations in colour

  • Topics: Pharmacology/Toxicology, Virology

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