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Nanomaterial Interfaces in Biology

Methods and Protocols

  • Book
  • © 2013

Overview

Editors:
  1. Paolo Bergese

    1. Dipto di Ingegneria Meccanica, Università degli Studi di Brescia, Brescia, Italy

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    You can also search for this editor in PubMed   Google Scholar

  2. Kimberly Hamad-Schifferli

    1. Dept of Mechanical Engineering, Massachusetts Institute of Technology, Cambridge, USA

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 1025)

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Table of contents (22 protocols)

  1. Front Matter

    Pages i-xi
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  2. Making Bio–Nano Interfaces

    1. Front Matter

      Pages 1-1
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    2. Preparation of 2 nm Gold Nanoparticles for In Vitro and In Vivo Applications

      • Daniel F. Moyano, Bradley Duncan, Vincent M. Rotello
      Pages 3-8

    3. DNA Conjugation to Nanoparticles

      • Sunho Park
      Pages 9-18

    4. Conjugation of Nanoparticles to Proteins

      • Marie-Eve Aubin-Tam
      Pages 19-27

    5. Water-Solubilization and Functionalization of Semiconductor Quantum Dots

      • Christina M. Tyrakowski, Adela Isovic, Preston T. Snee
      Pages 29-45

    6. Synthesizing and Modifying Peptides for Chemoselective Ligation and Assembly into Quantum Dot-Peptide Bioconjugates

      • W. Russ Algar, Juan B. Blanco-Canosa, Rachel L. Manthe, Kimihiro Susumu, Michael H. Stewart, Philip E. Dawson et al.
      Pages 47-73

    7. Reliable Methods for Silica Coating of Au Nanoparticles

      • Isabel Pastoriza-Santos, Luis M. Liz-Marzán
      Pages 75-93

    8. Surface Modifications by Polymers for Biomolecule Conjugation

      • Laura Sola, Marina Cretich, Francesco Damin, Marcella Chiari
      Pages 95-107

    9. Functionalization Protocols of Silicon Micro/Nano-mechanical Biosensors

      • Francesca Frascella, Carlo Ricciardi
      Pages 109-115

  3. Characterizing Bio–Nano Interfaces

    1. Front Matter

      Pages 117-117
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    2. Stability and Aggregation Assays of Nanoparticles in Biological Media

      • James Chen Yong Kah
      Pages 119-126

    3. Electrochemical Measurements of DNA Melting on Surfaces

      • Irina Belozerova, Dongbiao Ge, Rastislav Levicky
      Pages 127-136

    4. Formation and Characterization of the Nanoparticle–Protein Corona

      • Marco P. Monopoli, Andrzej S. Pitek, Iseult Lynch, Kenneth A. Dawson
      Pages 137-155

    5. Electrophoretic Implementation of the Solution-Depletion Method for Measuring Protein Adsorption, Adsorption Kinetics, and Adsorption Competition Among Multiple Proteins in Solution

      • Hyeran Noh, Naris Barnthip, Purnendu Parhi, Erwin A. Vogler
      Pages 157-166

    6. Hyperspectral Microscopy for Characterization of Gold Nanoparticles in Biological Media and Cells for Toxicity Assessment

      • Christin Grabinski, John Schlager, Saber Hussain
      Pages 167-178

    7. Immunocytochemistry, Electron Tomography, and Energy Dispersive X-ray Spectroscopy (EDXS) on Cryosections of Human Cancer Cells Doped with Stimuli Responsive Polymeric Nanogels Loaded with Iron Oxide Nanoparticles

      • Roberto Marotta, A. Falqui, A. Curcio, A. Quarta, Teresa Pellegrino
      Pages 179-198

  4. Implementing Bio–Nano Interfaces

    1. Front Matter

      Pages 199-199
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    2. Zwitterion Siloxane to Passivate Silica Against Nonspecific Protein Adsorption

      • Zaki G. Estephan, Joseph B. Schlenoff
      Pages 201-205

    3. Preparation and Characterization of DNA Block Copolymer Assemblies Loaded with Nanoparticles

      • Xi-Jun Chen, Robert J. Hickey, So-Jung Park
      Pages 207-224
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Keywords

About this book

The intersection of nanotechnology with biology has given rise to numerous ideas for new ways to use nanotechnology for biological applications. Nanomaterials possess unique size- and material-dependent properties which make them attractive for improving regular biomedical fields, such as drug delivery, imaging, therapy, and diagnostics. Divided into three convenient sections, Nanomaterial Interfaces in Biology: Methods and Protocols covers protocols describing synthesis, fabrication, and construction of bio-nanomaterial interfaces, characterization protocols of bio-nanomaterial interfaces, and applications which utilize the bio-nanomaterial interfaces. Written in the highly successful Methods in Molecular Biology series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.

 

Authoritative and accessible Nanomaterial Interfaces in Biology: Methods and Protocols will serve the new and emerging scientific community, enabling new capabilities and technologies that were not previously possible in medicine and biology.

Editors and Affiliations

  • Dipto di Ingegneria Meccanica, Università degli Studi di Brescia, Brescia, Italy

    Paolo Bergese

  • Dept of Mechanical Engineering, Massachusetts Institute of Technology, Cambridge, USA

    Kimberly Hamad-Schifferli

Bibliographic Information

  • Book Title: Nanomaterial Interfaces in Biology

  • Book Subtitle: Methods and Protocols

  • Editors: Paolo Bergese, Kimberly Hamad-Schifferli

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-62703-462-3

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2013

  • Hardcover ISBN: 978-1-62703-461-6Published: 06 August 2013

  • Softcover ISBN: 978-1-4939-6007-1Published: 23 August 2016

  • eBook ISBN: 978-1-62703-462-3Published: 13 August 2013

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 293

  • Number of Illustrations: 20 b/w illustrations, 53 illustrations in colour

  • Topics: Biotechnology, Nanotechnology

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