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Corneal Regenerative Medicine

Methods and Protocols

  • Book
  • © 2013

Overview

Editors:
  1. Bernice Wright

    1. School of Pharmacy, University of Reading, Reading, United Kingdom

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    You can also search for this editor in PubMed   Google Scholar

  2. Che J Connon

    1. School of Pharmacy, University of Reading, Reading, United Kingdom

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1014)

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Table of contents (15 protocols)

  1. Front Matter

    Pages i-xii
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  2. Perspectives

    1. Front Matter

      Pages 1-1
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    2. Limbal Epithelial Cell Therapy: Past, Present, and Future

      • Tor Paaske Utheim
      Pages 3-43

    3. The Artificial Cornea

      • Naresh Polisetti, Mohammad Mirazul Islam, May Griffith
      Pages 45-52

  3. Corneal Stem Cell Enrichment and Characterization

    1. Front Matter

      Pages 53-53
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    2. Clonal Analysis of Limbal Epithelial Stem Cell Populations

      • Ursula Schlötzer-Schrehardt
      Pages 55-64

    3. The Use of Bromodeoxyuridine Incorporation Assays to Assess Corneal Stem Cell Proliferation

      • Ashley M. Crane, Sanjoy K. Bhattacharya
      Pages 65-70

    4. Enrichment of Human Corneal Epithelial Stem/Progenitor Cells by Magnetic Bead Sorting Using SSEA4 as a Negative Marker

      • Martin N. Nakatsu, Sophie X. Deng
      Pages 71-77

    5. Limbal Epithelial Stem Cell Identification Using Immunoblotting Analysis

      • Bernice Wright, Che J. Connon
      Pages 79-99

  4. Corneal Cell Culture

    1. Front Matter

      Pages 101-101
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    2. The Culture of Limbal Epithelial Cells

      • Tor Paaske Utheim, Torstein Lyberg, Sten Ræder
      Pages 103-129

    3. The Culture of Limbal Stromal Cells and Corneal Endothelial Cells

      • Naresh Polisetti, Nancy C. Joyce
      Pages 131-139

  5. Development of the Artificial Cornea

    1. Front Matter

      Pages 141-141
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    2. The Formation of a Tissue-Engineered Cornea Using Plastically Compressed Collagen Scaffolds and Limbal Stem Cells

      • Shengli Mi, Che J. Connon
      Pages 143-155

    3. Fabrication of a Human Recombinant Collagen-Based Corneal Substitute Using Carbodiimide Chemistry

      • Mohammad Mirazul Islam, May Griffith, Kimberley Merrett
      Pages 157-164

    4. Fabrication of a Corneal-Limbal Tissue Substitute Using Silk Fibroin

      • Laura J. Bray, Karina A. George, Shuko Suzuki, Traian V. Chirila, Damien G. Harkin
      Pages 165-178

    5. Cultivation of Limbal Epithelial Cells on Electrospun Poly (lactide-co-glycolide) Scaffolds for Delivery to the Cornea

      • Pallavi Deshpande, Charanya Ramachandran, Virender S. Sangwan, Sheila MacNeil
      Pages 179-185

    6. The Growth and Delivery of Mesenchymal and Limbal Stem Cells Using Copolymer Polyamide 6/12 Nanofiber Scaffolds

      • Vladimir Holan, Eliska Javorkova, Peter Trosan
      Pages 187-199

    7. Encapsulation and Culture of Mammalian Cells Including Corneal Cells in Alginate Hydrogels

      • Nicola C. Hunt, Liam M. Grover
      Pages 201-210

    8. In Vivo Confocal Microscopy of the Cornea to Assess Tissue Regenerative Response After Biomaterial Implantation in Humans

      • Neil Lagali, May Griffith, Per Fagerholm
      Pages 211-223
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Keywords

About this book

Continuous regeneration of the cornea is necessary to maintain this tissue in the transparent state that is essential for vision. Therapy for repair of the damaged anterior cornea is currently addressed through the transplantation of donor corneas or the delivery of limbal epithelial stem cells (LESC) to the ocular surface using amniotic membrane (AM) as a supporting scaffold. Research on the bioengineering of corneal equivalents as replacement tissue is underway to develop viable corneal prosthetics. Corneal Regenerative Medicine: Methods and Protocols provides a concise overview of essential techniques in the field of corneal regenerative medicine, highlighting novel strategies to guide the management of key therapies within this area of medicine. Divided into four convenient sections, topics include the identification, characterisation and cultivation of LESC, as well as the investigation of biopolymers used as the basis for corneal substitutes. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.

 

Authoritative and easily accessible, Corneal Regenerative Medicine: Methods and Protocols covers the fundamental techniques useful for both the laboratory and clinical settings.  

Editors and Affiliations

  • School of Pharmacy, University of Reading, Reading, United Kingdom

    Bernice Wright, Che J Connon

Bibliographic Information

  • Book Title: Corneal Regenerative Medicine

  • Book Subtitle: Methods and Protocols

  • Editors: Bernice Wright, Che J Connon

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-62703-432-6

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2013

  • Hardcover ISBN: 978-1-62703-431-9Published: 21 May 2013

  • Softcover ISBN: 978-1-4939-6272-3Published: 23 August 2016

  • eBook ISBN: 978-1-62703-432-6Published: 21 May 2013

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XII, 230

  • Number of Illustrations: 23 b/w illustrations, 33 illustrations in colour

  • Topics: Molecular Medicine, Ophthalmology

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