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  • © 2013

Jasmonate Signaling

Methods and Protocols

  • Combines cutting-edge omics protocols with more common reductionist approaches to the study of jasmonate signaling pathways
  • Features clear, step-by-step methodologies provided by experts in the field
  • Includes key tips and vital implementation advice to ensure successful results
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 1011)

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Table of contents (26 protocols)

  1. Front Matter

    Pages i-xiii
  2. Physiology

    1. Front Matter

      Pages 1-1
    2. Phenotyping Jasmonate Regulation of Senescence

      • Martin A. Seltmann, Susanne Berger
      Pages 3-11
    3. Characterizing Jasmonate Regulation of Male Fertility in Arabidopsis

      • Bryan Thines, Ajin Mandaokar, John Browse
      Pages 13-23
    4. Phenotyping Jasmonate Regulation of Root Growth

      • Fabian Kellermeier, Anna Amtmann
      Pages 25-32
  3. Environment

    1. Front Matter

      Pages 33-33
    2. Bioassays for Assessing Jasmonate-Dependent Defenses Triggered by Pathogens, Herbivorous Insects, or Beneficial Rhizobacteria

      • Saskia C. M. Van Wees, Johan A. Van Pelt, Peter A. H. M. Bakker, Corné M. J. Pieterse
      Pages 35-49
    3. Pseudomonas syringae Infection Assays in Arabidopsis

      • Jian Yao, John Withers, Sheng Yang He
      Pages 63-81
  4. Molecules

    1. Front Matter

      Pages 111-111
    2. Profiling of Jasmonic Acid-Related Metabolites and Hormones in Wounded Leaves

      • Yusuke Jikumaru, Mitsunori Seo, Hideyuki Matsuura, Yuji Kamiya
      Pages 113-122
    3. Cell-Specific Detection of Jasmonates by Means of an Immunocytological Approach

      • Bettina Hause, Kati Mielke, Susanne Forner
      Pages 135-144
    4. Jasmonic Acid–Amino Acid Conjugation Enzyme Assays

      • Martha L. Rowe, Paul E. Staswick
      Pages 145-157
    5. Yeast Two-Hybrid Analysis of Jasmonate Signaling Proteins

      • Amparo Pérez Cuéllar, Laurens Pauwels, Rebecca De Clercq, Alain Goossens
      Pages 173-185

About this book

It is now well established that jasmonates, originally identified as the major component of jasmine scent, play a universal role in the plant kingdom and are involved in the regulation of diverse aspects of plant biology, including growth, development, metabolism, and interaction with the environment.  In Jasmonate Signaling: Methods and Protocols, experts in the field aim to unite powerful emerging omics platforms with a number of key reductionist approaches to form a comprehensive collection of tools and protocols. The detailed chapters in this book embrace physiological, environmental, molecular, omics, and bioinformatics approaches that allow dissecting jasmonate actions in the model species Arabidopsis thaliana or in other plants.  Written in the highly successful Methods in Molecular Biology series format, chapters feature introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, along with tips on troubleshooting and avoiding known pitfalls.

 

Authoritative and cutting-edge, Jasmonate Signaling: Methods and Protocols will empower interested researchers to dissect all steps of jasmonate signaling and the processes they modulate.

Editors and Affiliations

  • Department of Plant Systems Biology, VIB, Gent, Belgium

    Alain Goossens, Laurens Pauwels

Bibliographic Information

  • Book Title: Jasmonate Signaling

  • Book Subtitle: Methods and Protocols

  • Editors: Alain Goossens, Laurens Pauwels

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-62703-414-2

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC 2013

  • Hardcover ISBN: 978-1-62703-413-5Published: 25 April 2013

  • Softcover ISBN: 978-1-4939-6013-2Published: 23 August 2016

  • eBook ISBN: 978-1-62703-414-2Published: 08 July 2014

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XIII, 347

  • Number of Illustrations: 23 b/w illustrations, 33 illustrations in colour

  • Topics: Plant Sciences, Plant Biochemistry

Buy it now

Buying options

eBook USD 84.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 159.00
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 109.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access