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  • © 2013

Vascular Proteomics

Methods and Protocols

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 1000)

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Table of contents (16 protocols)

  1. Front Matter

    Pages i-xi
  2. Vascular Proteomics

    • Maria G. Barderas, Fernando Vivanco, Gloria Alvarez-Llamas
    Pages 1-20
  3. Laser Microdissection and Saturation Labeling DIGE Method for the Analysis of Human Arteries

    • Fernando de la Cuesta, Gloria Alvarez-Llamas, Aroa S. Maroto, Maria G. Barderas, Fernando Vivanco
    Pages 21-32
  4. Use of TOF-SIMS in Vascular Biology

    • Sebastián Mas, Raúl Pérez, Jesús Egido
    Pages 33-43
  5. Proteomic Analysis of Vascular Smooth Muscle Cells with S- and R-Enantiomers of Atenolol by iTRAQ and LC-MS/MS

    • Jianhua Zhang, Jiahua Shi, Benedict Jia Hong Lee, Liwei Chen, Kee Yang Tan, Xiaoling Tang et al.
    Pages 45-52
  6. Phosphoproteomic Analysis of Aortic Endothelial Cells Activated by Oxidized Phospholipids

    • Alejandro Zimman, Judith A. Berliner, Thomas G. Graeber
    Pages 53-69
  7. Characterization of Membrane and Cytosolic Proteins of Erythrocytes

    • Gloria Alvarez-Llamas, Fernando de la Cuesta, Maria G. Barderas, Irene Zubiri, Maria Posada-Ayala, Fernando Vivanco
    Pages 71-80
  8. Characterization and Analysis of Human Arterial Tissue Secretome by 2-DE and nLC-MS/MS

    • Fernando de la Cuesta, Maria G. Barderas, Enrique Calvo, Irene Zubiri, Aroa S. Maroto, Juan Antonio Lopez et al.
    Pages 81-90
  9. Identification of Novel Biomarkers of Abdominal Aortic Aneurysms by 2D-DIGE and MALDI-MS from AAA-Thrombus-Conditioned Media

    • Roxana Martinez-Pinna, Juan Antonio Lopez, Priscila Ramos-Mozo, Luis M. Blanco-Colio, Emilio Camafeita, Enrique Calvo et al.
    Pages 91-101
  10. Metabolites Secreted by Human Atherothrombotic Aneurysm

    • Michal Ciborowski, Coral Barbas
    Pages 103-113
  11. Quantitative Proteomics Analysis of High-Density Lipoproteins by Stable 18O-Isotope Labeling

    • Elena Burillo, Jesus Vazquez, Inmaculada Jorge
    Pages 139-156
  12. Unraveling Biomarkers of Abdominal Aortic Aneurisms by iTRAQ Analysis of Depleted Plasma

    • Enrique Calvo, Roxana Martínez-Pinna, Priscila Ramos-Mozo, C. Pastor-Vargas, Emilio Camafeita, Jesús Egido et al.
    Pages 157-166
  13. Absolute Quantitation of Proteins in Human Blood by Multiplexed Multiple Reaction Monitoring Mass Spectrometry

    • Andrew J. Percy, Andrew G. Chambers, Carol E. Parker, Christoph H. Borchers
    Pages 167-189
  14. Multiple Reaction Monitoring (MRM) of Plasma Proteins in Cardiovascular Proteomics

    • Verónica M. Dardé, Maria G. Barderas, Fernando Vivanco
    Pages 191-199
  15. Back Matter

    Pages 221-230

About this book

Proteomics is a rapidly expanding investigation platform in cardiovascular medicine. Driven by major improvements in mass spectrometry (MS) instrumentation and data analysis, the proteomics field has flourished in recent years particularly in the study of complex diseases. These recent advances are characterized by the development of quantitative MS-based methods that promoted the field from primarily identifying proteins to also providing measurements of relative changes in protein levels between different cell states. This progress is reflected in the application of proteomic techniques to vascular pathology. Vascular Proteomics: Methods and Protocols provides up-to-date methods and protocols for the analysis of arteries, cells, lipoproteins, body fluids, and metabolites, with a particular focus on MS-based methods of protein and peptide quantification. Written in the successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.

 

Authoritative and easily accessible, Vascular Proteomics: Methods and Protocols is a representative selection of methods that can be a useful resource for experienced proteomics practitioners as well as newcomers interested in becoming acquainted with the practice of proteomic techniques for cardiovascular research.

Editors and Affiliations

  • Depto. Immunología, Fundación Jiménez Díaz, Madrid, Spain

    Fernando Vivanco

Bibliographic Information

  • Book Title: Vascular Proteomics

  • Book Subtitle: Methods and Protocols

  • Editors: Fernando Vivanco

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-62703-405-0

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2013

  • Hardcover ISBN: 978-1-62703-404-3Published: 14 April 2013

  • Softcover ISBN: 978-1-4939-6001-9Published: 23 August 2016

  • eBook ISBN: 978-1-62703-405-0Published: 13 April 2013

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 230

  • Topics: Protein Science, Cardiology

Buy it now

Buying options

eBook USD 84.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 139.00
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 109.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access