Overview
- Includes cutting-edge methods and protocols for microsatellite markers in the MiMB standard format
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
- Includes supplementary material: sn.pub/extras
Part of the book series: Methods in Molecular Biology (MIMB, volume 1006)
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Table of contents (21 protocols)
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Discovery and Development of Microsatellites
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Amplification and Visualization
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Automated Capillary Sequencers
Keywords
About this book
Microsatellites or simple sequence repeats (SSRs) have become the markers of choice for a variety of molecular studies because of their versatility, operational flexibility, and lower cost than other marker systems. Microsatellites: Methods and Protocols brings together experts in the field to cover this significant area of research. Broken in to four convenient parts, this volume delves into classical and modern methods for the discovery and development of microsatellite markers, descriptions of amplification and visualization of SSRs, automated capillary sequencers that are widely used for fragment analysis, as well as a variety of methods for the analysis of data obtained by the use of microsatellites. Written for the highly successful Methods in Molecular Biology™ series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and invaluable, Microsatellites: Methods and Protocols aims at researchers that need detailed protocols for incorporating microsatellite markers into their projects and expert scientists looking to expand their knowledge of SSRs discovery, use, and analysis.
Editors and Affiliations
Bibliographic Information
Book Title: Microsatellites
Book Subtitle: Methods and Protocols
Editors: Stella K. Kantartzi
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-62703-389-3
Publisher: Humana Totowa, NJ
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media, LLC 2013
Hardcover ISBN: 978-1-62703-388-6Published: 02 April 2013
Softcover ISBN: 978-1-4939-6309-6Published: 23 August 2016
eBook ISBN: 978-1-62703-389-3Published: 02 April 2013
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: XI, 339
Topics: Human Genetics