Necrosis

1. Front Matter

   Pages i-xi

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2. Analysis of Necrosis In Vitro

   1. Front Matter

      Pages 1-1

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   2. Navigation to the Graveyard-Induction of Various Pathways of Necrosis and Their Classification by Flow Cytometry

      • Christina Janko, Luis Munoz, Ricardo Chaurio, Christian Maueröder, Christian Berens, Kirsten Lauber et al.

      Pages 3-15

   3. Time-Lapse Imaging of Necrosis

      • Fredrik Wållberg, Tencho Tenev, Pascal Meier

      Pages 17-29

   4. Activity Assays for Receptor-Interacting Protein Kinase 1:A Key Regulator of Necroptosis

      • Jenny L. Maki, Alexei Degterev

      Pages 31-42

   5. Fluorescent Biosensors for the Detection of HMGB1 Release

      • Isabelle Martins, Oliver Kepp, Laurie Menger, Mickäel Michaud, Sandy Adjemian, Abdul Qader Sukkurwala et al.

      Pages 43-56

3. Alternative Cell Death Modalities

   1. Front Matter

      Pages 57-57

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   2. Methods for the Study of Entosis

      • Qiang Sun, Michael Overholtzer

      Pages 59-66

   3. Analysis of Pyroptosis in Bacterial Infection

      • Lia Danelishvili, Luiz E. Bermudez

      Pages 67-73

4. Analysis of Cell Death in Mammalian Tissues

   1. Front Matter

      Pages 75-75

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   2. Analysis of Cell Death by Electron Microscopy

      • S. Burattini, E. Falcieri

      Pages 77-89

   3. Histological Analysis of Neurodegeneration in the Mouse Brain

      • Hiroo Yamaguchi, Jie Shen

      Pages 91-113

   4. Quantitation of Acute Necrosis After Experimental Myocardial Infarction

      • Xin-Yi Yeap, Shirley Dehn, Jeremy Adelman, Jeremy Lipsitz, Edward B. Thorp

      Pages 115-133

   5. Immunohistological Tools to Discriminate Apoptotic and Necrotic Cell Death in the Skin

      • Marion C. Bonnet

      Pages 135-142

5. Cell Death in Nonmammalian Model Systems

   1. Front Matter

      Pages 143-143

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   2. Identification and Characterization of Programmed Cell Death Markers in Bacterial Models

      • Daniel J. Dwyer, Jonathan A. Winkler

      Pages 145-159

   3. Quantification of Genetically Controlled Cell Death in Budding Yeast

      • Xinchen Teng, J. Marie Hardwick

      Pages 161-170

   4. Necrosis in C. elegans

      • Matt Crook, Avni Upadhyay, Wendy Hanna-Rose

      Pages 171-182

   5. Monitoring the Clearance of Apoptotic and Necrotic Cells in the Nematode Caenorhabditis elegans

      • Zao Li, Nan Lu, Xiangwei He, Zheng Zhou

      Pages 183-202

   6. Modeling Calcium-Overload Mediated Necrosis in Drosophila

      • Kai Liu, Yuhong Li, Lei Liu

      Pages 203-213

Cell death is an essential process in development, and a major contributor to a wide range of human diseases.  Three major classifications of cell death, apoptosis, autophagic cell death and necrosis, have been described for years, and the existence of many more forms of cell death is now accepted. In, Necrosis: Methods and Protocols experts in the field provide a wide range of methods and techniques for the study of necrosis in vitro and in vivo. These include methods and techniques for the analysis of necrosis in mammalian cells, characterization of alternative forms of cell death: entosis and pyroptosis, and analysis of cell death in non-mammalian model sytems and mammalian tissues, including chapters on skin, brain, and heart. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.
 
 
Authoritative and practical, Necrosis: Methods and Protocols describes techniques  in an easy to follow manner, with details so that beginners can succeed with challenging techniques.

• Biochemical
• apoptosis in vivo
• apoptotic cell death
• autophagic cell death
• entosis
• mammalian cells
• mammalian tissues
• necrosis
• pyroptosis
• signaling pathways
• Cell Cycle Analysis
• Apoptosis

• Department of Biology, Boston University, Boston, USA

  Kimberly McCall

• Brookline, USA

  Charles Klein

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