Skip to main content
SpringerLink
Log in
Book cover

Olfactory Receptors

Methods and Protocols

  • Book
  • © 2013

Overview

Editors:
  1. Chiquito J. Crasto

    1. , Department of Genetics, University of Alabama, Birmingham, Birmingham, USA

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Collects chapters from significant contributors to the field of olfactory receptor research
  • Features easy to follow methods and protocols, ready for the lab
  • Includes expert tips and key implementation advice to ensure successful results
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 1003)

This is a preview of subscription content, log in via an institution to check access.

Access this book

Log in via an institution
eBook USD 89.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 119.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 169.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access

Licence this eBook for your library

Institutional subscriptions

Table of contents (18 protocols)

  1. Front Matter

    Pages i-xv
    Download chapter PDF

  2. Knowledge Dissemination of Olfactory Receptors

    1. Front Matter

      Pages 1-1
      Download chapter PDF

    2. Databases in SenseLab for the Genomics, Proteomics, and Function of Olfactory Receptors

      • Luis N. Marenco, Gautam Bahl, Lorra Hyland, Jing Shi, Rixin Wang, Peter C. Lai et al.
      Pages 3-22

    3. HORDE: Comprehensive Resource for Olfactory Receptor Genomics

      • Tsviya Olender, Noam Nativ, Doron Lancet
      Pages 23-38

    4. Identification of Olfactory Receptor Genes from Mammalian Genome Sequences

      • Yoshihito Niimura
      Pages 39-49

  3. Theoretical Assessments of Olfactory Receptor Structure and Function

    1. Front Matter

      Pages 51-51
      Download chapter PDF

    2. Molecular Modelling of Odorant/Olfactory Receptor Complexes

      • Landry Charlier, Jérémie Topin, Claire A. de March, Peter C. Lai, Chiquito J. Crasto, Jerome Golebiowski
      Pages 53-65

    3. The Electrical Properties of Olfactory Receptors in the Development of Biological Smell Sensors

      • Eleonora Alfinito, Jeremy Pousset, Lino Reggiani
      Pages 67-83

    4. A Hit Map-Based Statistical Method to Predict Best Ligands for Orphan Olfactory Receptors: Natural Key Odorants Versus “Lock Picks”

      • Dietmar Krautwurst, Matthias Kotthoff
      Pages 85-97

  4. Expression Systems for Olfactory Receptors

    1. Front Matter

      Pages 99-99
      Download chapter PDF

    2. A Robust, Rapid, and Simple Method of Producing Olfactory Receptors Using Commercial E. coli Cell-Free Systems

      • Karolina Corin, Brian Cook, Shuguang Zhang
      Pages 101-108

    3. Human Olfactory Receptors: Recombinant Expression in the Baculovirus/Sf9 Insect Cell System, Functional Characterization, and Odorant Identification

      • Valéry Matarazzo, Catherine Ronin
      Pages 109-122

    4. Single Cell RT-PCR Identification of Odorant Receptors Expressed by Olfactory Neurons

      • Bettina Malnic
      Pages 123-132

    5. Next-Generation Sequencing of the Human Olfactory Receptors

      • Joel D. Mainland, Jason R. Willer, Hiroaki Matsunami, Nicholas Katsanis
      Pages 133-147

    6. Deciphering Activation of Olfactory Receptors Using Heterologous Expression in Saccharomyces cerevisiae and Bioluminescence Resonance Energy Transfer

      • Guenhaël Sanz, Edith Pajot-Augy
      Pages 149-160

    7. Olfactory Receptors in Human Airway Epithelia

      • Xiaoling Gu, Yehuda Ben-Shahar
      Pages 161-169

  5. Functional Analysis of Olfactory Receptors

    1. Front Matter

      Pages 171-171
      Download chapter PDF

    2. Functional Characterization of Insect Olfactory Receptor Neurons Through In Vivo Approaches

      • Michel Renou, Philippe Lucas
      Pages 173-186

    3. Functional Assay of Mammalian and Insect Olfactory Receptors Using Xenopus Oocytes

      • Charles W. Luetje, Andrew S. Nichols, Ana Castro, Benjamin L. Sherman
      Pages 187-202

    4. Pharmacology of Mammalian Olfactory Receptors

      • Richard S. Smith, Zita Peterlin, Ricardo C. Araneda
      Pages 203-209
Back to top

Keywords

About this book

Despite the best efforts of many and despite landmark discoveries and experimental ingenuity, challenges in the pursuit of research related to olfactory receptors (ORs) continue to exist.  In Olfactory Receptors: Methods and Protocols, experts in the field contribute chapters that serve to address these challenges.  The volume consists of several sections: knowledge dissemination of ORs, theoretical assessments of OR structure and function, as well as development and use of expression systems and experimental functional analysis. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 

Concise and practical,  Olfactory Receptors: Methods and Protocols seeks to aid researchers in furthering the knowledge of olfaction and moving us ever closer to the thrilling discoveries that will follow.

Editors and Affiliations

  • , Department of Genetics, University of Alabama, Birmingham, Birmingham, USA

    Chiquito J. Crasto

Bibliographic Information

  • Book Title: Olfactory Receptors

  • Book Subtitle: Methods and Protocols

  • Editors: Chiquito J. Crasto

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-62703-377-0

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC 2013

  • Hardcover ISBN: 978-1-62703-376-3Published: 14 April 2013

  • Softcover ISBN: 978-1-4939-6202-0Published: 23 August 2016

  • eBook ISBN: 978-1-62703-377-0Published: 13 April 2013

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XV, 259

  • Topics: Human Genetics, Animal Genetics and Genomics

Publish with us

Policies and ethics

Back to top

Search

Navigation