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Cellular and Subcellular Nanotechnology

Methods and Protocols

  • Book
  • © 2013

Overview

Editors:
  1. Volkmar Weissig

    1. College of Pharmacy, Dept. Pharmaceutical Sciences, Midwestern University, Glendale, USA

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    You can also search for this editor in PubMed   Google Scholar

  2. Tamer Elbayoumi

    1. , Department of Pharmaceutical Sciences, Midwestern University College of Pharmac, Glendale, USA

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  3. Mark Olsen

    1. , Department of Pharmaceutical Sciences, Midwestern University College of Pharmac, Glendale, USA

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Provides detailed applications of the latest nanotechnologies on the cellular and subcellular level for the purpose of probing and manipulating cellular function
  • Supplies easy to follow, readily reproducible laboratory protocols
  • Contains expert tips for troubleshooting and avoiding known pitfalls
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 991)

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Table of contents (32 protocols)

  1. Front Matter

    Pages i-xvi
    Download chapter PDF

  2. Nanoparticle-GFP “Chemical Nose” Sensor for Cancer Cell Identification

    • Daniel F. Moyano, Vincent M. Rotello
    Pages 1-8

  3. A Method to Map Spatiotemporal pH Changes in a Multicellular Living Organism Using a DNA Nanosensor

    • Sunaina Surana, Yamuna Krishnan
    Pages 9-23

  4. A Simple Method to Visualize and Assess the Integrity of Lysosomal Membrane in Mammalian Cells Using a Fluorescent Dye

    • Syed K. Sohaebuddin, Liping Tang
    Pages 25-31

  5. Gold Nanoparticle as a Marker for Precise Localization of Nano-objects Within Intracellular Sub-domains

    • Valeriy Lukyanenko, Vadim Salnikov
    Pages 33-39

  6. Immunoisolation of Nanoparticles Containing Endocytic Vesicles for Drug Quantitation

    • Ari Nowacek, Irena Kadiu, JoEllyn McMillan, Howard E. Gendelman
    Pages 41-46

  7. Methods for Isolation and Identification of Nanoparticle-Containing Subcellular Compartments

    • Ari Nowacek, Irena Kadiu, JoEllyn McMillan, Howard E. Gendelman
    Pages 47-55

  8. Permeabilization of Cell Membrane for Delivery of Nano-objects to Cellular Sub-domains

    • Valeriy Lukyanenko
    Pages 57-63

  9. A Method to Encapsulate Molecular Cargo Within DNA Icosahedra

    • Dhiraj Bhatia, Saikat Chakraborty, Shabana Mehtab, Yamuna Krishnan
    Pages 65-80

  10. Delivery of Plasmid DNA to Mammalian Cells Using Polymer–Gold Nanorod Assemblies

    • James Ramos, Huang-Chiao Huang, Kaushal Rege
    Pages 81-91

  11. Lipophilic-Formulated Gold Porphyrin Nanoparticles for Chemotherapy

    • Puiyan Lee, Kenneth K. Y. Wong
    Pages 93-98

  12. Mitochondria-Specific Nano-Emulsified Therapy for Myocardial Protection Against Doxorubicin-Induced Cardiotoxicity

    • Amy Faulk, Volkmar Weissig, Tamer Elbayoumi
    Pages 99-112

  13. Formation of Pit-Spanning Phospholipid Bilayers on Nanostructured Silicon Dioxide Surfaces for Studying Biological Membrane Events

    • Indriati Pfeiffer, Michael Zäch
    Pages 113-125

  14. Characterization of Nanoparticle–Lipid Membrane Interactions Using QCM-D

    • Rickard Frost, Sofia Svedhem
    Pages 127-137

  15. Single-Cell Nanosurgery

    • Maxwell B. Zeigler, Daniel T. Chiu
    Pages 139-148

  16. Single Quantum Dot Imaging in Living Cells

    • Jerry C. Chang, Sandra J. Rosenthal
    Pages 149-162

  17. Fabrication of Fluorescent Silica Nanoparticles with Aggregation-Induced Emission Luminogens for Cell Imaging

    • Sijie Chen, Jacky W. Y. Lam, Ben Zhong Tang
    Pages 163-169

  18. Monitoring the Degradation of Reduction-Sensitive Gene Carriers with Fluorescence Spectroscopy and Flow Cytometry

    • Constantin Hozsa, Miriam Breunig, Achim Göpferich
    Pages 171-184

  19. Quantification of Intracellular Mitochondrial Displacements in Response to Nanomechanical Forces

    • Yaron R. Silberberg, Andrew E. Pelling
    Pages 185-193

  20. Imaging Select Mammalian Organelles Using Fluorescent Microscopy: Application to Drug Delivery

    • Paul D. R. Dyer, Arun K. Kotha, Marie W. Pettit, Simon C. W. Richardson
    Pages 195-209
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Keywords

About this book

In Cellular and Subcellular Nanotechnology: Methods and Protocols expert researchers in the field detail the most recent advances which have been made in utilizing the enormous potential of nanotechnology for probing, imaging and manipulating life on a cellular and subcellular level. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

 

Authoritative and Practical, Cellular and Subcellular Nanotechnology: Methods and Protocols seeks to aid scientists in the further study of applying nanotechnology to all areas of biomedical sciences.

Editors and Affiliations

  • College of Pharmacy, Dept. Pharmaceutical Sciences, Midwestern University, Glendale, USA

    Volkmar Weissig

  • , Department of Pharmaceutical Sciences, Midwestern University College of Pharmac, Glendale, USA

    Tamer Elbayoumi, Mark Olsen

Bibliographic Information

  • Book Title: Cellular and Subcellular Nanotechnology

  • Book Subtitle: Methods and Protocols

  • Editors: Volkmar Weissig, Tamer Elbayoumi, Mark Olsen

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-62703-336-7

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2013

  • Hardcover ISBN: 978-1-62703-335-0Published: 02 April 2013

  • Softcover ISBN: 978-1-4939-6243-3Published: 23 August 2016

  • eBook ISBN: 978-1-62703-336-7Published: 02 April 2013

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XVI, 370

  • Topics: Biotechnology, Nanotechnology

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