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Plant Meiosis

Methods and Protocols

  • Book
  • © 2013

Overview

Editors:
  1. Wojciech P. Pawlowski

    1. , Dept. of Plant Breeding & Genetics, Cornell University, Ithaca, USA

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  2. Mathilde Grelon

    1. , Centre de Versailles-Grignon, Inst National de la Recherche Agronomiqu, Versailles, France

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  3. Susan Armstrong

    1. School of Biosciences, The University of Birmingham, Birmingham, United Kingdom

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Details methods for molecular cytogenetics and chromosome analysis
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB)

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Table of contents (22 protocols)

  1. Front Matter

    Pages i-xv
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  2. Cytological Techniques for Light Microscopy

    1. Front Matter

      Pages 1-1
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    2. Spreading and Fluorescence In Situ Hybridization of Male and Female Meiocyte Chromosomes from Arabidopsis thaliana for Cytogenetical Analysis

      • Susan Armstrong
      Pages 3-11

    3. Analysis of Plant Meiotic Chromosomes by Chromosome Painting

      • Martin A. Lysak, Terezie Mandáková
      Pages 13-24

    4. Using Sequential Fluorescence and Genomic In Situ Hybridization (FISH and GISH) to Distinguish the A and C Genomes in Brassica napus

      • Elaine C. Howell, Susan Armstrong
      Pages 25-34

    5. Labeling Meiotic Chromosomes in Maize with Fluorescence In Situ Hybridization

      • Zhi Gao, Fangpu Han, Tatiana V. Danilova, Jonathan C. Lamb, Patrice S. Albert, James A. Birchler
      Pages 35-43

    6. Examining Female Meiocytes of Maize by Confocal Microscopy

      • Philippa Barrell, Ueli Grossniklaus
      Pages 45-52

    7. Three-Dimensional Acrylamide Fluorescence In Situ Hybridization for Plant Cells

      • Elizabeth S. Howe, Shaun P. Murphy, Hank W. Bass
      Pages 53-66

    8. Analyzing Maize Meiotic Chromosomes with Super-Resolution Structured Illumination Microscopy

      • Chung-Ju Rachel Wang
      Pages 67-78

    9. Live Imaging of Chromosome Dynamics

      • Moira J. Sheehan, R. Kelly Dawe, Wojciech P. Pawlowski
      Pages 79-92

    10. Immunolocalization of Meiotic Proteins in Brassicaceae: Method 1

      • Liudmila A. Chelysheva, Laurie Grandont, Mathilde Grelon
      Pages 93-101

    11. Immunolocalization of Meiotic Proteins in Arabidopsis thaliana: Method 2

      • Susan Armstrong, Kim Osman
      Pages 103-107

    12. Immunolocalization Protocols for Visualizing Meiotic Proteins in Arabidopsis thaliana: Method 3

      • Xiaohui Yang, Li Yuan, Christopher A. Makaroff
      Pages 109-118

    13. A Time Course for the Analysis of Meiotic Progression in Arabidopsis thaliana

      • Susan Armstrong
      Pages 119-123

    14. Analyzing Meiotic Chromosomes in Rice

      • Zhukuan Cheng
      Pages 125-134

    15. Analyzing Meiosis in Barley

      • James D. Higgins
      Pages 135-144

  3. Cytological Techniques for Electron Microscopy

    1. Front Matter

      Pages 145-145
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    2. Preparing SC Spreads with RNs for EM Analysis

      • Lorinda K. Anderson, Stephen M. Stack
      Pages 147-158

    3. Analysis of the Synaptonemal Complex in Brassica Using TEM

      • Susan Armstrong
      Pages 159-166

    4. Preparing Thin Sections of Meiotic Nuclei for Transmission Electron Microscopy

      • Ljudmilla Timofejeva
      Pages 167-174
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Keywords

About this book

Meiosis is one of the most critical processes in eukaryotes, required for continuation of species and generation of new variation.  In plants, meiotic recombination is by far the most important source of genetic variation.  In Plant Meiosis: Methods and Protocols, expert researchers in the field detail methods for molecular cytogenetics and chromosome analysis in plants.  These state-of -the-art protocols allow studying the organization and behavior of the genetic material in a wide range of both model and crop species.  Written in the highly successful Methods in Molecular Biology™series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

 

 Authoritative and practical, Plant Meiosis: Methods and Protocols provides and extensive list of protocols developed and used in a number of laboratories at the cutting edge of meiosis and chromosome research.

Editors and Affiliations

  • , Dept. of Plant Breeding & Genetics, Cornell University, Ithaca, USA

    Wojciech P. Pawlowski

  • , Centre de Versailles-Grignon, Inst National de la Recherche Agronomiqu, Versailles, France

    Mathilde Grelon

  • School of Biosciences, The University of Birmingham, Birmingham, United Kingdom

    Susan Armstrong

About the editors

Meiosis is one of the most critical processes in eukaryotes, required for continuation of species and generation of new variation.  In plants, meiotic recombination is by far the most important source of genetic variation.  In Plant Meiosis: Methods and Protocols, expert researchers in the field detail methods for molecular cytogenetics and chromosome analysis in plants.  These methods and protocols allows for understand the organization and behavior of the genetic material in a wide range of both model and crop species. extensive list of protocols developed and used in a number of laboratories at the cutting edge of meiosis and chromosome research.  Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

 

 

Authoritative and practical, Plant Meiosis: Methods and Protocols provides and extensive list of protocols developed and used in a number of laboratories at the cutting edge of meiosis and chromosome research.

Bibliographic Information

  • Book Title: Plant Meiosis

  • Book Subtitle: Methods and Protocols

  • Editors: Wojciech P. Pawlowski, Mathilde Grelon, Susan Armstrong

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-62703-333-6

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2013

  • Hardcover ISBN: 978-1-62703-332-9Published: 05 April 2013

  • Softcover ISBN: 978-1-4939-5972-3Published: 23 August 2016

  • eBook ISBN: 978-1-62703-333-6Published: 05 April 2013

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XV, 238

  • Topics: Plant Sciences, Plant Genetics and Genomics

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