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Target Identification and Validation in Drug Discovery

Methods and Protocols

  • Book
  • © 2013

Overview

Editors:
  1. Jurgen Moll

    1. Director, Department of Pharmacology, Boehringer Ingelheim RCV GmbH & Co KG, Vienna, Austria

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  2. Riccardo Colombo

    1. Project and Team Leader, Cell Biology, Nerviano Medical Sciences, Nerviano, Italy

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB)

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Table of contents (22 protocols)

  1. Front Matter

    Pages i-xv
    Download chapter PDF

  2. Background

    1. Front Matter

      Pages 1-1
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    2. The Path to Oncology Drug Target Validation: An Industry Perspective

      • Marta Cortés-Cros, Tobias Schmelzle, Volker M. Stucke, Francesco Hofmann
      Pages 3-13

  3. DNA as a Tool to Modulate Drug Targets

    1. Front Matter

      Pages 15-15
      Download chapter PDF

    2. Identification of Aptamers as Specific Binders and Modulators of Cell-Surface Receptor Activity

      • Henning Ulrich, Carsten Wrenger
      Pages 17-39

    3. The Design and Structure–Functional Properties of DNA-Based Immunomodulatory Sequences

      • Nikolai V. Kuznetsov
      Pages 41-56

  4. RNA Interference: From Design to Data Analysis

    1. Front Matter

      Pages 57-57
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    2. siRNA Design Principles and Off-Target Effects

      • Sebastian Petri, Gunter Meister
      Pages 59-71

    3. Western Blot Evaluation of siRNA Delivery by pH-Responsive Peptides

      • Wanling Liang, A. James Mason, Jenny K. W. Lam
      Pages 73-87

    4. High-Throughput RNAi Screening for the Identification of Novel Targets

      • Meredith C. Henderson, David O. Azorsa
      Pages 89-95

    5. Integration of RNAi and Small Molecule Screens to Identify Targets for Drug Development

      • Konstantinos Drosopoulos, Spiros Linardopoulos
      Pages 97-104

    6. CellProfiler and KNIME: Open Source Tools for High Content Screening

      • Martin Stöter, Antje Niederlein, Rico Barsacchi, Felix Meyenhofer, Holger Brandl, Marc Bickle
      Pages 105-122

    7. PARP Inhibition as a Prototype for Synthetic Lethal Screens

      • Xuesong Liu
      Pages 123-137

  5. Protein-Focused Technologies

    1. Front Matter

      Pages 139-139
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    2. Structure-Based Target Druggability Assessment

      • Jean-Yves Trosset, Nicolas Vodovar
      Pages 141-164

    3. Validating Pharmacological Disruption of Protein–Protein Interactions by Acceptor Photobleaching FRET Imaging

      • Janos Roszik, Gábor Tóth, János SzöllÅ‘si, György Vereb
      Pages 165-178

    4. Systematic Analysis of Complex Signal Transduction Pathways Using Protein Fragment Complementation Assays

      • Thomas I. Koblizek, Ann Siehoff, Anthony Pitt
      Pages 179-185

    5. Reverse Phase Protein Microarrays and Their Utility in Drug Development

      • Maria Isabella Sereni, Mariaelena Pierobon, Roberto Angioli, Emanuel F. Petricoin III, Mitchell J. Frederick
      Pages 187-214

  6. Innovative Cell Culture Techniques to Mimic Tissue Microenvironments

    1. Front Matter

      Pages 215-215
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    2. A Cell Culture System That Mimics Chronic Lymphocytic Leukemia Cells Microenvironment for Drug Screening and Characterization

      • Alessandro Natoni, Michael O’Dwyer, Corrado Santocanale
      Pages 217-226
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Keywords

About this book

The major reason for the elevated costs of drug development in the pharmaceutical industry is the high attrition rate. In Target Identification and Validation in Drug Discovery: Methods and Protocols, expert researchers in the field detail many of the methods which are now commonly used to identify and validate new target. These include methods and approaches covering biochemical, cell based, in vivo models and translational methods. Chapters also include selected case reports that demonstrate the integration of these technologies to real life experiences and to demonstrate the multiple use of more than one technology to increase knowledge on a specific target. These Written in the highly successful Methods in Molecular Biologyâ„¢ series format, the chapters include the kind of detailed description and implementation advice that is crucial for getting optimal results in the laboratory.

 

Thorough and intuitive, Target Identification and Validation in Drug Discovery: Methods and Protocols contains a comprehensive list of essential methods and clear protocols to follow.

Editors and Affiliations

  • Director, Department of Pharmacology, Boehringer Ingelheim RCV GmbH & Co KG, Vienna, Austria

    Jurgen Moll

  • Project and Team Leader, Cell Biology, Nerviano Medical Sciences, Nerviano, Italy

    Riccardo Colombo

Bibliographic Information

  • Book Title: Target Identification and Validation in Drug Discovery

  • Book Subtitle: Methods and Protocols

  • Editors: Jurgen Moll, Riccardo Colombo

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-62703-311-4

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2013

  • Hardcover ISBN: 978-1-62703-310-7Published: 23 February 2013

  • Softcover ISBN: 978-1-4939-6278-5Published: 19 August 2016

  • eBook ISBN: 978-1-62703-311-4Published: 26 February 2013

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XV, 371

  • Topics: Pharmacology/Toxicology, Medicinal Chemistry

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