Editors:
- Covers many aspects of the tissue-protective actions of erythropoietin (EPO) and other cytokines
- Collects methods of models where cytokine molecules can be characterized
- Presents notes on troubleshooting and avoiding known pitfalls
- Includes supplementary material: sn.pub/extras
Part of the book series: Methods in Molecular Biology (MIMB)
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Table of contents (19 protocols)
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Front Matter
About this book
While many cytokines are known for their inflammatory action, there is a growing interest in the tissue-protective effects of some cytokines. The prototypic tissue-protective cytokine is EPO. Initially described as neuro-protective, it is beneficial in animal models of ischemic and other types of injury. Scientists had to overcome the notion that EPO had only erythropoietic actions, was only produced by the kidney, and that its receptor was only present in erythroid progenitor cells. The use of in vitro and in vivo disease models was essential to demonstrate the protective effects of EPO. Reproducible models will be needed for the further study of the mechanism of action of EPO and for the identification of other tissue-protective cytokines.
 In Tissue-Protective Cytokines: Methods and Protocols, expert researchers in the field detail the key models that have been used to characterize the tissue-protective actions of cytokines. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, provide step-by-step laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.
 Thorough and intuitive, Tissue Protective Cytokines: Methods and Protocols aids scientists in continuing to study tissue-protection that will be a new field of interest of cytokine biology, both in discovering novel actions of known cytokines and in developing new drugs.
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Editors and Affiliations
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Medical School, Brighton and Sussex, Falmer, United Kingdom
Pietro Ghezzi
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Leiden University Medical Center, Leiden, Netherlands
Anthony Cerami
Bibliographic Information
Book Title: Tissue-Protective Cytokines
Book Subtitle: Methods and Protocols
Editors: Pietro Ghezzi, Anthony Cerami
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-62703-308-4
Publisher: Humana Totowa, NJ
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media, LLC 2013
Hardcover ISBN: 978-1-62703-307-7Published: 02 March 2013
Softcover ISBN: 978-1-4939-6244-0Published: 23 August 2016
eBook ISBN: 978-1-62703-308-4Published: 02 March 2013
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: XI, 328
Topics: Biochemistry, general, Human Physiology