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  • © 2013

Immune Homeostasis

Methods and Protocols

  • Focuses on experimental techniques for measuring and analyzing immune cell dynamics
  • Emphasizes lymphocyte programmed cell deaths in different contexts
  • Features expert tips and key implementation advice to guarantee successful results
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 979)

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Table of contents (16 protocols)

  1. Front Matter

    Pages i-xi
  2. Analyses of Programmed Cell Death in Dendritic Cells

    • Min Chen, Lily Huang, Jin Wang
    Pages 51-63
  3. Detection of Necrosis by Release of Lactate Dehydrogenase Activity

    • Francis Ka-Ming Chan, Kenta Moriwaki, María José De Rosa
    Pages 65-70
  4. Homeostatic Proliferation of Mature T Cells

    • Christopher E. Martin, Kwesi Frimpong-Boateng, Darina S. Spasova, John C. Stone, Charles D. Surh
    Pages 81-106
  5. Quantitating Lymphocyte Homeostasis In Vivo in Humans Using Stable Isotope Tracers

    • Liset Westera, Yan Zhang, Kiki Tesselaar, José A. M. Borghans, Derek C. Macallan
    Pages 107-131
  6. Real-Time Quantitative (RQ-)PCR Approach to Quantify the Contribution of Proliferation to B Lymphocyte Homeostasis

    • Menno C. van Zelm, Magdalena A. Berkowska, Mirjam van der Burg, Jacques J. M. van Dongen
    Pages 133-145
  7. Molecular Measurement of T Cell Receptor Excision Circles

    • Heather E. Lynch, Gregory D. Sempowski
    Pages 147-159
  8. Isolation of RNA and the Synthesis and Amplification of cDNA from Antigen-Specific T Cells for Genome-Wide Expression Analysis

    • R. Anthony Barnitz, Sabrina Imam, Kathleen Yates, W. Nicholas Haining
    Pages 161-173
  9. Flow Cytometric Measurement of SLAM-Associated Protein and X-Linked Inhibitor of Apoptosis

    • Rebecca A. Marsh, Jack J. Bleesing, Alexandra H. Filipovich
    Pages 189-197
  10. Back Matter

    Pages 213-215

About this book

Many questions remain unresolved as researchers continue to characterize and define the nature of normal immune homeostasis and determine how these processes are dysregulated in immunodeficiency, as well as in autoimmune and lymphoproliferative disorders.  Immune Homeostasis: Methods and Protocols focuses on experimental techniques for measuring and analyzing immune cell dynamics, with a particular emphasis on examining lymphocyte programmed cell death in different contexts.  With contributions by leading experts in the field, the collection examines detailed protocols for studying various pathways of apoptosis and necrosis in different types of hematopoietic cells, both in vitro and in vivo, methods for studying the maintenance of lymphocyte populations in the steady-state or following infectious challenges in both mice and humans, and technical insights into state-of-the-art genomics tools, among other topics.  Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 

Valuable and easy to use, Immune Homeostasis: Methods and Protocols serves as an important toolkit for basic and clinical scientists interested in examining various aspects of immune homeostasis in both normal and disease-related contexts in order to further study the dynamic processes that contribute to homeostasis of the immune system.

Editors and Affiliations

  • of the Health Sciences, Department of Pharmacology, Uniformed Services University, Bethesda, USA

    Andrew L. Snow

  • NIAID, Laboratory of Immunology, National Institutes of Health, Bethesda, USA

    Michael J. Lenardo

Bibliographic Information

  • Book Title: Immune Homeostasis

  • Book Subtitle: Methods and Protocols

  • Editors: Andrew L. Snow, Michael J. Lenardo

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-62703-290-2

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2013

  • Hardcover ISBN: 978-1-62703-289-6Published: 09 February 2013

  • Softcover ISBN: 978-1-4939-5968-6Published: 23 August 2016

  • eBook ISBN: 978-1-62703-290-2Published: 11 February 2013

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 215

  • Topics: Immunology

Buy it now

Buying options

eBook USD 89.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 119.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 169.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access