Overview
- Focuses on leading, current approaches in the study of dopamine within a variety of biological systems
- Features detailed protocols that have been tested in laboratories around the world
- Includes expert tips and key implementation advice to ensure successful results
Part of the book series: Methods in Molecular Biology (MIMB, volume 964)
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Table of contents (17 protocols)
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Front Matter
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Biochemical, Proteomic, and Computational Tools
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Front Matter
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Cellular Imaging
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Front Matter
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Genetic Manipulation in Cells and Organisms
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Front Matter
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Electrochemical, Physiological, and Behavioral Analysis
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Front Matter
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Keywords
About this book
Dopamine, a catecholamine transmitter, plays a number of vital physiological roles in the brain and body, and, in recent years, studies on the role of dopamine in disease have opened new avenues of research and discovery. In Dopamine: Methods and Protocols, experts and key figures within the field provide detailed protocols on leading approaches in the study of dopamine within biological systems. Divided into sections on cellular/biochemical, imaging, genetics, and electrophysiology, this collections includes protocols for bioluminescence and fluorescence imaging, receptor immunoprecipitation and proteomic analysis, creation and characterization of a mouse model of Parkinson’s disease, real time measurement of dopamine in the brain, and modeling signal transduction in silico. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective subjects, lists of materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and convenient, Dopamine: Methods and Protocols serves as an ideal guide for students and experts alike, as well as for anyone interested in exploring the vast and crucial field of dopamine research.
Reviews
From the book reviews:
“Designed for active researchers ranging from students to established scientists, the book is edited by a proteomics researcher working the in the area of dopamine and nicotine receptor protein interaction networks. … this book adds a collection of methods for contemporary techniques that should prove useful to researchers wishing to apply these approaches to their work.” (Beth Levant, Doody’s Book Reviews, May, 2013)Editors and Affiliations
Bibliographic Information
Book Title: Dopamine
Book Subtitle: Methods and Protocols
Editors: Nadine Kabbani
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-62703-251-3
Publisher: Humana Totowa, NJ
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media, LLC 2013
Hardcover ISBN: 978-1-62703-250-6Published: 08 January 2013
Softcover ISBN: 978-1-4939-6333-1Published: 23 August 2016
eBook ISBN: 978-1-62703-251-3Published: 09 January 2013
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: XI, 312
Topics: Neurosciences, Neurobiology, Neurochemistry