Overview
- Includes cutting-edge methods and protocols
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
- Includes supplementary material: sn.pub/extras
Part of the book series: Methods in Molecular Biology (MIMB, volume 967)
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Table of contents (21 protocols)
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Front Matter
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JAK Protein Tyrosine Kinases
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Front Matter
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Signal Transducers and Activators of Transcription
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Front Matter
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Signal Transducers and Activators of Transcription (STAT)s
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Suppressor of Cytokine Signalling Proteins
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Front Matter
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Keywords
About this book
The receptor-associated JAK protein kinases and their substrates, the STAT transcriptional activators, transmit signals following cytokine and growth factor binding to receptors expressed on the cell surface, to result in specific transcriptional and cellular responses. Over the last two decades, the field has progressed from identification of the individual components through to an understanding of the activation and deactivation mechanisms, and the complex structural detail of the proteins involved. We now know that these pathways are important in many biological processes, including growth and development, hematopoiesis, and the innate and adaptive immune response. JAK-STAT Signalling: Methods and Protocols provides detailed methodologies for examining many aspects of the pathway. Divided into four sections, topics include JAK and STAT specific approaches, the negative regulators of the pathway (SOCS proteins), and the production and crystallization of JAK and STAT proteins, among others. Written in the successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.
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Authoritative and easily accessible, JAK-STAT Signalling: Methods and Protocols will be of use not only to those working in the area but also to new investigators who are led to delve into the complexities of JAK and STAT responses.
Editors and Affiliations
Bibliographic Information
Book Title: JAK-STAT Signalling
Book Subtitle: Methods and Protocols
Editors: Sandra E. Nicholson, Nicos A. Nicola
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-62703-242-1
Publisher: Humana Totowa, NJ
eBook Packages: Springer Protocols
Copyright Information: Springer Science + Business Media New York 2013
Hardcover ISBN: 978-1-62703-241-4Published: 08 January 2013
Softcover ISBN: 978-1-4939-5979-2Published: 23 August 2016
eBook ISBN: 978-1-62703-242-1Published: 09 January 2013
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: XI, 320
Topics: Cell Biology, Cytokines and Growth Factors