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p53 Protocols

  • Book
  • © 2013

Overview

Editors:
  1. Sumitra Deb

    1. Massey Cancer Center, Department of Biochemistry, Virginia Commonwealth University, Richmond, USA

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  2. Swati Palit Deb

    1. Massey Cancer Center, Department of Biochemistry, Virginia Commonwealth University, Richmond, USA

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Fully updated with cutting-edge additions to the popular first edition
  • Provides step-by-step reproducible laboratory protocols
  • Contains tips on troubleshooting and avoiding known pitfalls
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 962)

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Table of contents (20 protocols)

  1. Front Matter

    Pages i-x
    Download chapter PDF

  2. Detecting and Quantifying p53 Isoforms at mRNA Level in Cell Lines and Tissues

    • Marie P. Khoury, Virginie Marcel, Kenneth Fernandes, Alexandra Diot, David P. Lane, Jean-Christophe Bourdon
    Pages 1-14

  3. Detecting p53 Isoforms at Protein Level

    • Virginie Marcel, Marie P. Khoury, Kenneth Fernandes, Alexandra Diot, David P. Lane, Jean-Christophe Bourdon
    Pages 15-29

  4. Autophagy, Senescence, and Apoptosis

    • Rachel W. Goehe, Molly L. Bristol, Eden N. Wilson, David A. Gewirtz
    Pages 31-48

  5. p53 and Cell Cycle Effects After DNA Damage

    • Emir Senturk, James J. Manfredi
    Pages 49-61

  6. p53 Ubiquitination and Proteasomal Degradation

    • Ian M. Love, Dingding Shi, Steven R. Grossman
    Pages 63-73

  7. Identification of p53 in Mitochondria

    • Angelina V. Vaseva, Ute M. Moll
    Pages 75-84

  8. Identification of Novel Mutant p53 Interacting Proteins by Proteomic Analysis

    • Sumitra Deb, Paul R. Graves
    Pages 85-94

  9. Identification of Small Molecules Affecting p53-MDM2/MDMX Interaction by Fluorescence Polarization

    • Qi Zhang, Hua Lu
    Pages 95-111

  10. Determine the Effect of p53 on Chemosensitivity

    • Emir Senturk, James J. Manfredi
    Pages 113-125

  11. Measurement of Chemosensitivity and Growth Rate in p53 Expressing Cells

    • Mahesh Ramamoorthy, Catherine Vaughan, Sumitra Deb, Swati Palit Deb
    Pages 127-133

  12. Mutant p53 in Cell Adhesion and Motility

    • W. Andrew Yeudall, Katharine H. Wrighton, Sumitra Deb
    Pages 135-146

  13. Use of the DNA Fiber Spreading Technique to Detect the Effects of Mutant p53 on DNA Replication

    • Rebecca A. Frum, Sumitra Deb, Swati Palit Deb
    Pages 147-155

  14. Generation of p53-Deficient Induced Pluripotent Stem Cells from Mouse Embryo Fibroblasts

    • Evguenia M. Alexandrova, Ute M. Moll
    Pages 157-164

  15. p53 Actions on MicroRNA Expression and Maturation Pathway

    • Hiroshi I. Suzuki, Kohei Miyazono
    Pages 165-181

  16. Isolation and Characterization of Murine Multipotent Lung Stem Cells

    • Venkat S. Gadepalli, Catherine Vaughan, Raj R. Rao
    Pages 183-191

  17. Generation of p53 Knock-down Cell Lines

    • Catherine Vaughan, Swati Palit Deb, Sumitra Deb
    Pages 193-199

  18. ChIP for Identification of p53 Responsive DNA Promoters

    • Jun-Ming Liao, Hua Lu
    Pages 201-210

  19. ChIP-on-chip to Identify Mutant p53 Targets

    • Frauke Goeman, Giulia Fontemaggi, Giovanni Blandino
    Pages 211-226

  20. ChIP Sequencing to Identify p53 Targets

    • Catherine Vaughan, Brad Windle, Sumitra Deb
    Pages 227-236
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Keywords

About this book

The second edition of p53 Protocols expands upon the first edition with progress in p53 research since the publication of the first edition. In p53 Protocols, Second Edition expert researchers in the field detail many of the methods that address the challenging questions of the p53 field.  These methods include the identification of the target genes and binding partners of gain of function p53 mutants, methods to determine stress response, autophagy or senescence induced by p53, cell cycle analysis, analysis of different phases of DNA replication modified by p53, and generation of pluripotent stem cells.  Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

 

Authoritative and practical, p53 Protocols, Second Edition seeks to aid scientists in the further study into p53 and other tumor suppressors.

Editors and Affiliations

  • Massey Cancer Center, Department of Biochemistry, Virginia Commonwealth University, Richmond, USA

    Sumitra Deb, Swati Palit Deb

Bibliographic Information

  • Book Title: p53 Protocols

  • Editors: Sumitra Deb, Swati Palit Deb

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-62703-236-0

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2013

  • Hardcover ISBN: 978-1-62703-235-3Published: 13 November 2012

  • Softcover ISBN: 978-1-4939-5933-4Published: 23 August 2016

  • eBook ISBN: 978-1-62703-236-0Published: 13 November 2012

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 2

  • Number of Pages: X, 238

  • Topics: Protein Science

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