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Calcium-Binding Proteins and RAGE

From Structural Basics to Clinical Applications

  • Book
  • © 2013

Overview

Editors:
  1. Claus W. Heizmann

    1. Div of Clinical Chem & Biochem, Department of Pediatrics, University of Zürich, Zürich, Switzerland

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  • Underlines the diagnostic and clinical importance of this family of proteins in human diseases
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 963)

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Table of contents (25 protocols)

  1. Front Matter

    Pages i-xiv
    Download chapter PDF

  2. Intracellular Ca2+-Measurements and Cation Interactions with EF-Hand Proteins

    1. Front Matter

      Pages 1-1
      Download chapter PDF

  3. Intracellular Ca2+- Measurements and Cation Interactions with EF—Hand Proteins

    1. Measurement of Intracellular Ca2+ Concentration in Single Cells Using Ratiometric Calcium Dyes

      • Sonal Srikanth, Yousang Gwack
      Pages 3-14

    2. Divers Models of Divalent Cation Interaction to Calcium-Binding Proteins: Techniques and Anthology

      • Jos A. Cox
      Pages 15-35

    3. Probing Ca2+-Binding Capability of Viral Proteins with the EF-Hand Motif by Grafting Approach

      • Yubin Zhou, Shenghui Xue, Yanyi Chen, Jenny J. Yang
      Pages 37-53

  4. Expression, Purification, Biochemical, and Structural Analyses and Cellular Localization

    1. Front Matter

      Pages 55-55
      Download chapter PDF

    2. New Aspects of Calmodulin–Calmodulin Binding Domains Recognition

      • Emilie Audran, Rania Dagher, Sophie Gioria, Marie-Claude Kilhoffer, Jacques Haiech
      Pages 57-72

    3. Purification and Characterization of the Human Cysteine-Rich S100A3 Protein and Its Pseudo Citrullinated Forms Expressed in Insect Cells

      • Kenji Kizawa, Masaki Unno, Hidenari Takahara, Claus W. Heizmann
      Pages 73-86

    4. X-ray Structural Analysis of S100 Proteins

      • Günter Fritz
      Pages 87-97

    5. Purification and Stable Isotope Labeling of the Calcium- and Integrin-Binding Protein 1 for Structural and Functional NMR Studies

      • Hao Huang, Hans J. Vogel
      Pages 99-113

    6. Analysis of Calcium-Induced Conformational Changes in Calcium-Binding Allergens and Quantitative Determination of Their IgE Binding Properties

      • Nuria Parody, Miguel Ángel Fuertes, Carlos Alonso, Yago Pico de Coaña
      Pages 115-125

    7. Longistatin, an EF-Hand Ca2+-Binding Protein from Vector Tick: Identification, Purification, and Characterization

      • Anisuzzaman, M. Khyrul Islam, M. Abdul Alim, Naotoshi Tsuji
      Pages 127-146

    8. Super-Resolution Microscopy of the Neuronal Calcium-Binding Proteins Calneuron-1 and Caldendrin

      • Johannes Hradsky, Marina Mikhaylova, Anna Karpova, Michael R. Kreutz, Werner Zuschratter
      Pages 147-169

  5. Interaction of Ca2+-Binding Proteins with Targets and Receptors/RAGE

    1. Front Matter

      Pages 171-171
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    2. NMR Studies of the Interaction of Calmodulin with IQ Motif Peptides

      • Steven M. Damo, Michael D. Feldkamp, Benjamin Chagot, Walter J. Chazin
      Pages 173-186

    3. Biochemical and Immunological Detection of Physical Interactions Between Penta-EF-Hand Protein ALG-2 and Its Binding Partners

      • Kanae Osugi, Hideki Shibata, Masatoshi Maki
      Pages 187-200

    4. Measuring Binding of S100 Proteins to RAGE by Surface Plasmon Resonance

      • Estelle Leclerc
      Pages 201-213

    5. Phage Display Selection of Peptides that Target Calcium-Binding Proteins

      • Stefan W. Vetter
      Pages 215-235

  6. RAGE-Meditated Cell Signaling and Pathology

    1. Front Matter

      Pages 237-237
      Download chapter PDF

  7. RAGE-Meditated CELL Signalling and Pathology

    1. RAGE-Mediated Cell Signaling

      • Ari Rouhiainen, Juha Kuja-Panula, Sarka Tumova, Heikki Rauvala
      Pages 239-263
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Keywords

About this book

A major direction in medical research leading to clinical applications  targets the regulation of intracellular calcium and the various human diseases associated with an altered homeostasis of this global second messenger. These diseases include, for example: cardiomyopathy, inflammation, brain disorders, diabetes and cancer. In Calcium-Binding Proteins and RAGE: from Structural Basics to Clinical Applications,expert researchers in the field detail many of the methods which are now commonly used to study calcium binding proteins. These methods and techniques, such as calcium-measurements, screening methods, clinical chemistry, and therapy, are generally applicable to many other areas of basic and medical research as well as to diagnostics. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

 

Authoritative and practical,Calcium-Binding Proteins and RAGE: from Structural Basics to Clinical Applications underlines the diagnostic and clinical importance of this family of proteins in human diseases and as drug targets.

Editors and Affiliations

  • Div of Clinical Chem & Biochem, Department of Pediatrics, University of Zürich, Zürich, Switzerland

    Claus W. Heizmann

Bibliographic Information

  • Book Title: Calcium-Binding Proteins and RAGE

  • Book Subtitle: From Structural Basics to Clinical Applications

  • Editors: Claus W. Heizmann

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-62703-230-8

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2013

  • Hardcover ISBN: 978-1-62703-229-2Published: 08 January 2013

  • Softcover ISBN: 978-1-4939-5976-1Published: 23 August 2016

  • eBook ISBN: 978-1-62703-230-8Published: 09 January 2013

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XIV, 424

  • Topics: Protein Science, Protein Structure

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