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Mass Spectrometry of Glycoproteins

Methods and Protocols

  • Book
  • © 2013

Overview

Editors:
  1. Jennifer J. Kohler

    1. Medical Center, Department of Biochemistry, University of Texas Southwestern, Dallas, USA

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  2. Steven M. Patrie

    1. Medical Center, Dallas, University of Texas Southwestern, Dallas, USA

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Resource for those who work at the interface of glycobiology and mass spectrometry
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 951)

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Table of contents (22 protocols)

  1. Front Matter

    Pages i-xi
    Download chapter PDF

  2. Introduction to Glycosylation and Mass Spectrometry

    • Steven M. Patrie, Michael J. Roth, Jennifer J. Kohler
    Pages 1-17

  3. Enrichment and Isolation Methods

    1. Front Matter

      Pages 19-19
      Download chapter PDF

    2. Tandem Lectin Weak Affinity Chromatography for Glycoprotein Enrichment

      • Zhi Yuan Ma, Yuliya Skorobogatko, Keith Vosseller
      Pages 21-31

    3. CSC Technology: Selective Labeling of Glycoproteins by Mild Oxidation to Phenotype Cells

      • Andreas Hofmann, Damaris Bausch-Fluck, Bernd Wollscheid
      Pages 33-43

    4. Use of Boronic Acid Nanoparticles in Glycoprotein Enrichment

      • Yawei Xu, Lijuan Zhang, Haojie Lu
      Pages 45-55

    5. Incorporation of Unnatural Sugars for the Identification of Glycoproteins

      • Balyn W. Zaro, Howard C. Hang, Matthew R. Pratt
      Pages 57-67

    6. Characterization of Membrane-Associated Glycoproteins Using Lectin Affinity Chromatography and Mass Spectrometry

      • Yashu Liu, Jintang He, David M. Lubman
      Pages 69-77

    7. Sialic Acid Capture-and-Release and LC-MSn Analysis of Glycopeptides

      • Jonas Nilsson, Göran Larson
      Pages 79-100

  4. Sample Preparation

    1. Front Matter

      Pages 101-101
      Download chapter PDF

    2. In-Solution Digestion of Glycoproteins for Glycopeptide-Based Mass Analysis

      • Eden P. Go, Kathryn R. Rebecchi, Heather Desaire
      Pages 103-111

    3. Nano-HPLC-MS of Glycopeptides Obtained After Nonspecific Proteolysis

      • Gerhild Zauner, Carolien A. M. Koeleman, André M. Deelder, Manfred Wuhrer
      Pages 113-127

  5. Separation Methods

    1. Front Matter

      Pages 129-129
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    2. Glycopeptide Enrichment for MALDI-TOF Mass Spectrometry Analysis by Hydrophilic Interaction Liquid Chromatography Solid Phase Extraction (HILIC SPE)

      • Pia Hønnerup Jensen, Simon Mysling, Peter Højrup, Ole Nørregaard Jensen
      Pages 131-144

    3. Separation and Identification of Glycoforms by Capillary Electrophoresis with Electrospray Ionization Mass Spectrometric Detection

      • Alina D. Zamfir, Corina Flangea, Alina Serb, Ana-Maria Zagrean, Andreas M. Rizzi, Eugen Sisu
      Pages 145-169

    4. Structural Separations by Ion Mobility-MS for Glycomics and Glycoproteomics

      • Larissa S. Fenn, John A. McLean
      Pages 171-194

  6. Quantitation Methods

    1. Front Matter

      Pages 195-195
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    2. Quantitative Analysis of Glycoprotein Glycans

      • Ron Orlando
      Pages 197-215

    3. Stable Isotope Labeling of N-Glycosylated Peptides by Enzymatic Deglycosylation for Mass Spectrometry-Based Glycoproteomics

      • Hiroyuki Kaji, Toshiaki Isobe
      Pages 217-227

    4. Approaches for Site Mapping and Quantification of O-Linked Glycopeptides

      • Peng Zhao, Stephanie H. Stalnaker, Lance Wells
      Pages 229-244
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Keywords

About this book

 Glycosylation is the most abundant post-translational modification of proteins.  Estimates vary widely, but a common assessment is that upwards of 50% of eukaryotic proteins are modified by some type of glycan. In Mass Spectrometry of Glycoproteins: Methods and Protocols, expert researchers in the field detail many of the methods that are now commonly used for glycoproteomics.  These methods and techniques include robust sample preparation techniques; advanced chromatographic strategies for improving dynamic range; state-of-the-art mass spectrometry instrumentation and associated ionization and fragmentation methods; and informatics tools used for identifying glycoproteins and characterizing the associated glycans. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

 

Authoritative and practical, Mass Spectrometry of Glycoproteins: Methods and Protocol  is  an essential resource for those who work at the interface of glycobiology and mass spectrometry.

Editors and Affiliations

  • Medical Center, Department of Biochemistry, University of Texas Southwestern, Dallas, USA

    Jennifer J. Kohler

  • Medical Center, Dallas, University of Texas Southwestern, Dallas, USA

    Steven M. Patrie

Bibliographic Information

  • Book Title: Mass Spectrometry of Glycoproteins

  • Book Subtitle: Methods and Protocols

  • Editors: Jennifer J. Kohler, Steven M. Patrie

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-62703-146-2

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC 2013

  • Hardcover ISBN: 978-1-62703-145-5Published: 08 January 2013

  • Softcover ISBN: 978-1-4939-6203-7Published: 23 August 2016

  • eBook ISBN: 978-1-62703-146-2Published: 09 January 2013

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 339

  • Topics: Protein Science, Mass Spectrometry

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