Overview
- Resource for those who work at the interface of glycobiology and mass spectrometry
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
Part of the book series: Methods in Molecular Biology (MIMB, volume 951)
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Table of contents (22 protocols)
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Enrichment and Isolation Methods
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Sample Preparation
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Separation Methods
Keywords
About this book
 Glycosylation is the most abundant post-translational modification of proteins. Estimates vary widely, but a common assessment is that upwards of 50% of eukaryotic proteins are modified by some type of glycan. In Mass Spectrometry of Glycoproteins: Methods and Protocols, expert researchers in the field detail many of the methods that are now commonly used for glycoproteomics. These methods and techniques include robust sample preparation techniques; advanced chromatographic strategies for improving dynamic range; state-of-the-art mass spectrometry instrumentation and associated ionization and fragmentation methods; and informatics tools used for identifying glycoproteins and characterizing the associated glycans. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.
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Authoritative and practical, Mass Spectrometry of Glycoproteins: Methods and Protocol  is an essential resource for those who work at the interface of glycobiology and mass spectrometry.Editors and Affiliations
Bibliographic Information
Book Title: Mass Spectrometry of Glycoproteins
Book Subtitle: Methods and Protocols
Editors: Jennifer J. Kohler, Steven M. Patrie
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-62703-146-2
Publisher: Humana Totowa, NJ
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media, LLC 2013
Hardcover ISBN: 978-1-62703-145-5Published: 08 January 2013
Softcover ISBN: 978-1-4939-6203-7Published: 23 August 2016
eBook ISBN: 978-1-62703-146-2Published: 09 January 2013
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: XI, 339
Topics: Protein Science, Mass Spectrometry