Mass Spectrometry of Glycoproteins

 Glycosylation is the most abundant post-translational modification of proteins.  Estimates vary widely, but a common assessment is that upwards of 50% of eukaryotic proteins are modified by some type of glycan. In Mass Spectrometry of Glycoproteins: Methods and Protocols, expert researchers in the field detail many of the methods that are now commonly used for glycoproteomics.  These methods and techniques include robust sample preparation techniques; advanced chromatographic strategies for improving dynamic range; state-of-the-art mass spectrometry instrumentation and associated ionization and fragmentation methods; and informatics tools used for identifying glycoproteins and characterizing the associated glycans. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

• Glycosylation
• associated ionization
• chromatographic strategies
• fragmentation methods
• glyco-specific computational tools
• glycoprotein
• mass spectrometry instrumentation

• Medical Center, Department of Biochemistry, University of Texas Southwestern, Dallas, USA

  Jennifer J. Kohler

• Medical Center, Dallas, University of Texas Southwestern, Dallas, USA

  Steven M. Patrie

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