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Nanotechnology for Nucleic Acid Delivery

Methods and Protocols

  • Book
  • © 2013

Overview

Editors:
  1. Manfred Ogris

    1. , Department of Pharmacy, Ludwig-Maximilians-University Munich, Munich, Germany

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    You can also search for this editor in PubMed   Google Scholar

  2. David Oupicky

    1. , Department of Pharmaceutical Sciences, Wayne State University, Detroit, USA

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 948)

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Table of contents (19 protocols)

  1. Front Matter

    Pages i-xii
    Download chapter PDF

  2. Toxicological Aspects for Nanomaterial in Humans

    • Maria Dusinska, Zuzana Magdolenova, Lise Marie Fjellsbø
    Pages 1-12

  3. Lactate Dehydrogenase Assay for Assessment of Polycation Cytotoxicity

    • Ladan Parhamifar, Helene Andersen, S. Moein Moghimi
    Pages 13-22

  4. Combined Fluorimetric Caspase 3/7 Assay and Bradford Protein Determination for Assessment of Polycation-Mediated Cytotoxicity

    • Anna K. Larsen, Arnaldur Hall, Henrik Lundsgart, S. Moein Moghimi
    Pages 23-33

  5. Anti-PEG IgM Production via a PEGylated Nano-Carrier System for Nucleic Acid Delivery

    • Masako Ichihara, Naoto Moriyoshi, Amr S. Abu Lila, Tatsuhiro Ishida, Hiroshi Kiwada
    Pages 35-47

  6. Near-Infrared Optical Imaging of Nucleic Acid Nanocarriers In Vivo

    • Claire Rome, Julien Gravier, Marie Morille, Gilles Divita, Anne-Laure Bolcato-Bellemin, Véronique Josserand et al.
    Pages 49-65

  7. Lipids for Nucleic Acid Delivery: Synthesis and Particle Formation

    • Michel Bessodes, Nathalie Mignet
    Pages 67-84

  8. Histidine-Rich Cationic Amphipathic Peptides for Plasmid DNA and siRNA Delivery

    • Antoine Kichler, A. James Mason, Arnaud Marquette, Burkhard Bechinger
    Pages 85-103

  9. Synthesis of Polyethylenimine-Based Nanocarriers for Systemic Tumor Targeting of Nucleic Acids

    • Wolfgang Rödl, David Schaffert, Ernst Wagner, Manfred Ogris
    Pages 105-120

  10. Synthesis of Bioreducible Polycations with Controlled Topologies

    • Ye-Zi You, Jun-Jie Yan, Zhi-Qiang Yu, David Oupicky
    Pages 121-132

  11. Lyophilization of Synthetic Gene Carriers

    • Julia Christina Kasper, Sarah Küchler, Wolfgang Friess
    Pages 133-147

  12. Surface- and Hydrogel-Mediated Delivery of Nucleic Acid Nanoparticles

    • Angela K. Pannier, Tatiana Segura
    Pages 149-169

  13. Layer-by-Layer Assembled Gold Nanoparticles for the Delivery of Nucleic Acids

    • Eva-Christina Wurster, Asmaa Elbakry, Achim Göpferich, Miriam Breunig
    Pages 171-182

  14. In Situ AFM Analysis Investigating Disassembly of DNA Nanoparticles and Nano-Films

    • Yi Zou, Lei Wan, Jenifer Blacklock, David Oupicky, Guangzhao Mao
    Pages 183-193

  15. Enhancing Nucleic Acid Delivery with Ultrasound and Microbubbles

    • Steven K. Cool, Bart Geers, Ine Lentacker, Stefaan C. De Smedt, Niek N. Sanders
    Pages 195-204

  16. Magnetic and Acoustically Active Microbubbles Loaded with Nucleic Acids for Gene Delivery

    • Dialechti Vlaskou, Christian Plank, Olga Mykhaylyk
    Pages 205-241

  17. Synthesis of Lipidic Magnetic Nanoparticles for Nucleic Acid Delivery

    • Yoshihisa Namiki
    Pages 243-250

  18. Lipopeptide Delivery of siRNA to the Central Nervous System

    • Mark D. Zabel
    Pages 251-262

  19. Flow Cytometry-Based Cell Type-Specific Assessment of Target Regulation by Pulmonary siRNA Delivery

    • Olivia M. Merkel, Leigh M. Marsh, Holger Garn, Thomas Kissel
    Pages 263-273

  20. Liver-Targeted Gene Delivery Through Retrograde Intrabiliary Infusion

    • Xuan Jiang, Yong Ren, John-Michael Williford, Zhiping Li, Hai-Quan Mao
    Pages 275-284
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Keywords

About this book

Nanotechnology and nucleic acid based therapies are two emerging fields in science whose combination has the potential to improve quality of life for patients suffering from various diseases that can so far only be treated in an unsatisfactory way. Nucleic acids offer the potential for highly selective treatment of such diseases or the highly specific modulation of gene expression with RNA interference. A key issue for successful nucleic acid therapies is the availability of a suitable delivery system. Here, the field of nanotechnology offers a multitude of possibilities to develop nanosized delivery vectors tailor-made for various local and systemic approaches. In Nanotechnology for Nucleic Acid Delivery: Methods and Protocols, experts in the field cover the area of nanoparticulate delivery of nucleic acids in terms of biosafety, particle synthesis as well as its application in cell culture. Written in the successful Methods in Molecular Biologyâ„¢ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.

 

Authoritative and easily accessible, Nanotechnology for Nucleic Acid Delivery: Methods and Protocols seeks to serve both professionals and novices with its well-honed methodologies.

Editors and Affiliations

  • , Department of Pharmacy, Ludwig-Maximilians-University Munich, Munich, Germany

    Manfred Ogris

  • , Department of Pharmaceutical Sciences, Wayne State University, Detroit, USA

    David Oupicky

Bibliographic Information

  • Book Title: Nanotechnology for Nucleic Acid Delivery

  • Book Subtitle: Methods and Protocols

  • Editors: Manfred Ogris, David Oupicky

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-62703-140-0

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC 2013

  • Hardcover ISBN: 978-1-62703-139-4Published: 16 October 2012

  • Softcover ISBN: 978-1-4939-5932-7Published: 23 August 2016

  • eBook ISBN: 978-1-62703-140-0Published: 16 October 2012

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XII, 286

  • Topics: Biotechnology, Nanotechnology

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