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Basic Cell Culture Protocols

  • Book
  • © 2013

Overview

Editors:
  1. Cheryl D. Helgason

    1. , Experimental Therapeutics, BC Cancer Agency, Vancouver, Canada

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  2. Cindy L. Miller

    1. STEMCELL Technologies, Inc., Vancouver, Canada

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Contains numerous new chapters that explore the most current areas of investigation as well as updated chapters from the previous edition
  • Includes clear and concise protocols, ready for the laboratory
  • Features expert tips and key implementation advice to ensure successful results

Part of the book series: Methods in Molecular Biology (MIMB, volume 946)

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Table of contents (33 protocols)

  1. Front Matter

    Pages i-xiv
    Download chapter PDF

  2. Detection of Mycoplasma Contaminations

    • Cord C. Uphoff, Hans G. Drexler
    Pages 1-13

  3. Eradication of Mycoplasma Contaminations

    • Cord C. Uphoff, Hans G. Drexler
    Pages 15-26

  4. STR DNA Typing of Human Cell Lines: Detection of Intra- and Interspecies Cross-Contamination

    • Wilhelm G. Dirks, Hans G. Drexler
    Pages 27-38

  5. Classical and Molecular Cytogenetic Analysis

    • Roderick A. F. MacLeod, Hans G. Drexler
    Pages 39-60

  6. Fluorescent In Situ Hybridization of DNA Probes in the Interphase and Metaphase Stages of the Cell Cycle

    • Linda A. Cannizzaro
    Pages 61-83

  7. The Development of T Lymphocytes in Fetal Thymus Organ Culture

    • Takeshi Nitta, Izumi Ohigashi, Yousuke Takahama
    Pages 85-102

  8. Generation, Isolation, and Engraftment of In Vitro-Derived Human T Cell Progenitors

    • Génève Awong, Juan Carlos Zúñiga-Pflücker
    Pages 103-113

  9. In Vitro Generation of Human T Regulatory Cells: Generation, Culture, and Analysis of FOXP3-Transduced T Cells

    • Alicia N. McMurchy, Megan K. Levings
    Pages 115-132

  10. Simultaneous Cloning and Selection of Hybridomas and Transfected Cell Lines in Semisolid Media

    • Bert Wognum, Tracy Lee
    Pages 133-149

  11. Isolation and Characterization of Mouse Side Population Cells

    • Aysegul V. Ergen, Mira Jeong, Kuanyin K. Lin, Grant A. Challen, Margaret A. Goodell
    Pages 151-162

  12. Stem Cell Identification by DyeCycle Violet Side Population Analysis

    • William G. Telford
    Pages 163-179

  13. Isolation and Characterization of Cancer Stem Cells In Vitro

    • Craig Gedye, Laurie Ailles
    Pages 181-204

  14. Ex Vivo Differentiation of Cord Blood Stem Cells into Megakaryocytes and Platelets

    • Nicolas Pineault, Amélie Robert, Valérie Cortin, Lucie Boyer
    Pages 205-224

  15. Generation and Characterization of Murine Alternatively Activated Macrophages

    • Shelley B. Weisser, Keith W. McLarren, Etsushi Kuroda, Laura M. Sly
    Pages 225-239

  16. Human Long-Term Culture Initiating Cell Assay

    • Min Liu, Cindy L. Miller, Connie J. Eaves
    Pages 241-256

  17. Long-Term Culture-Initiating Cell Assay for Mouse Cells

    • Stefan Woehrer, Cindy L. Miller, Connie J. Eaves
    Pages 257-266

  18. Colony Forming Cell Assays for Human Hematopoietic Progenitor Cells

    • Bert Wognum, Ning Yuan, Becky Lai, Cindy L. Miller
    Pages 267-283

  19. Studying Leukocyte Recruitment Under Flow Conditions

    • Sean A. Parsons, Christophe Jurzinsky, Susan L. Cuvelier, Kamala D. Patel
    Pages 285-300

  20. Generation and Establishment of Murine Adherent Cell Lines

    • Rouzanna Istvanffy, Robert A. J. Oostendorp
    Pages 301-314
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Keywords

About this book

At some point in their careers, virtually every scientist and technician, as well as many medical professionals, regardless of their area of specialization have a need to utilize cell culture systems. Updating and significantly expanding upon the previous editions, Basic Cell Culture Protocols, Fourth Edition provides the novice cell culturist with sufficient information to perform the basic techniques, to ensure the health and identity of their cell lines, and to be able to isolate and culture specialized primary cell types. The intent of this extensive volume is to generate a valuable resource containing clear methodologies pertinent to current areas of investigation, rather than attempting to educate cell culturists on specific cell types or organ systems.  Written in the highly successful Methods in Molecular Biology™, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 

Comprehensive and up-to-date, Basic Cell Culture Protocols, Fourth Edition compiles the essential techniques needed to approach this vital laboratory activity with full success.

Editors and Affiliations

  • , Experimental Therapeutics, BC Cancer Agency, Vancouver, Canada

    Cheryl D. Helgason

  • STEMCELL Technologies, Inc., Vancouver, Canada

    Cindy L. Miller

Bibliographic Information

  • Book Title: Basic Cell Culture Protocols

  • Editors: Cheryl D. Helgason, Cindy L. Miller

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-62703-128-8

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC 2013

  • Hardcover ISBN: 978-1-62703-127-1Published: 20 November 2012

  • Softcover ISBN: 978-1-4939-5652-4Published: 23 August 2016

  • eBook ISBN: 978-1-62703-128-8Published: 28 November 2012

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 4

  • Number of Pages: XIV, 550

  • Topics: Cell Biology, Cell Culture

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