Editors:
- Combines brand new protocols with heavily revised and improved methodologies
- Features lab-ready procedures with step-by-step instruction
- Includes expert tips and key implementation advice to ensure successful results
- Includes supplementary material: sn.pub/extras
Part of the book series: Methods in Molecular Biology (MIMB, volume 937)
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Table of contents (21 protocols)
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Front Matter
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Specialist Measurement Systems
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Front Matter
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Measurement of Ins(1,4,5)P3 and Ca2+ Release from Intracellular Stores
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Front Matter
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Measurement of Ins(1,4,5)P3 and Ca2+ Release Form Intracellular Stores
About this book
The regulation of intracellular Ca2+ has continued to be a powerful area of study since the publication of the first and second editions of Calcium Signaling Protocols, and the developments in the field have also, naturally, continued. With the third edition, expert contributors explore some of the exciting new molecular techniques that have both enabled new studies of intracellular Ca2+ regulation and provided much new information on processes. Comprised of five main section, the book covers theoretical and very simple suspension-based fluorimetric assays, specialist measurement systems, measurement of channel activity, measurement of store release, as well as specialist measurement techniques which include targeted probes, using G-protein chimeras to force Ca2+ signalling for screening, and genetically encoded sensors. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
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Updated and accessible, Calcium Signaling Protocols, Third Edition will be a valuable reference for all those contemplating a move into the study of intracellular Ca2+.Editors and Affiliations
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, Department of Cardiovascular Sciences, Leicester Royal Infirmary, Leicester, United Kingdom
David G. Lambert, Richard D. Rainbow
Bibliographic Information
Book Title: Calcium Signaling Protocols
Editors: David G. Lambert, Richard D. Rainbow
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-62703-086-1
Publisher: Humana Totowa, NJ
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media, LLC 2013
Hardcover ISBN: 978-1-62703-085-4Published: 26 September 2012
Softcover ISBN: 978-1-4939-5928-0Published: 23 August 2016
eBook ISBN: 978-1-62703-086-1Published: 25 September 2012
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 3
Number of Pages: XI, 360
Topics: Biochemistry, general