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  • © 2012

Membrane Protein Structure and Dynamics

Methods and Protocols

  • Lists the necessary materials and provides the needed background information for all experiments
  • Provides step-by-step detail essential fro reproducible results
  • Contains key notes and implementation advice from the experts
  • Includes supplementary material: sn.pub/extras
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 914)

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Table of contents (19 protocols)

  1. Front Matter

    Pages i-xi
  2. Experimental Techniques for Membrane Protein Structure Determination

    1. Front Matter

      Pages 1-1
    2. Crystallization of Membrane Proteins in Bicelles

      • Sayeh Agah, Salem Faham
      Pages 3-16
    3. Vapor Diffusion-Controlled Meso Crystallization of Membrane Proteins

      • J. Labahn, J. Kubicek, F. Schäfer
      Pages 17-24
    4. Use of NMR Saturation Transfer Difference Spectroscopy to Study Ligand Binding to Membrane Proteins

      • Rani Parvathy Venkitakrishnan, Outhiriaradjou Benard, Marianna Max, John L. Markley, Fariba M. Assadi-Porter
      Pages 47-63
    5. How to Investigate Interactions Between Membrane Proteins and Ligands by Solid-State NMR

      • Andrea Lakatos, Karsten Mörs, Clemens Glaubitz
      Pages 65-86
    6. Identifying and Measuring Transmembrane Helix–Helix Interactions by FRET

      • Damien Thévenin, Tzvetana Lazarova
      Pages 87-106
  3. Computational Methods for Prediction of Membrane Protein Structure and Dynamics

    1. Front Matter

      Pages 165-165
    2. Homology Model-Assisted Elucidation of Binding Sites in GPCRs

      • Anat Levit, Dov Barak, Maik Behrens, Wolfgang Meyerhof, Masha Y. Niv
      Pages 179-205
    3. Comparative Modeling of Lipid Receptors

      • Abby L. Parrill
      Pages 207-218
    4. Structure Prediction of G Protein-Coupled Receptors and Their Ensemble of Functionally Important Conformations

      • Ravinder Abrol, Adam R. Griffith, Jenelle K. Bray, William A. Goddard III
      Pages 237-254
    5. Identification of Motions in Membrane Proteins by Elastic Network Models and Their Experimental Validation

      • Basak Isin, Kalyan C. Tirupula, Zoltán N. Oltvai, Judith Klein-Seetharaman, Ivet Bahar
      Pages 285-317

About this book

Membrane proteins play key roles in numerous cellular processes, in particular mediating cell-to-cell communication and signaling events that lead to a multitude of biological effects. Membrane proteins have also been implicated in many critical diseases such as atherosclerosis, hypertension, diabetes and cancer. In Membrane Protein Structure Predictions Methods: Methods and Protocols, expert researcher in the field detail the advances in both experimental and computational approaches of the structure, dynamics and interactions of membrane proteins dividing the volume into two sections. The first section details the procedures used for measurements of structure and dynamics of membrane proteins. While the second section contains a survey of the computational methods that have played a critical role in membrane protein structure prediction as well as in providing atomic level insight into the mechanism of the dynamics of membrane receptors. Written in the highly successful Methods in Molecular Biologyâ„¢ series format, the chapters include the kind of detailed description and implementation advice that is crucial for getting optimal results in the laboratory.

Thorough and intuitive, Membrane Protein Structure Predicitons: Methods and Protocols seeks to aid scientists in the further study of membrane protein structure and function.

Editors and Affiliations

  • , Division of Immunology, Beckman Research Institute of the City o, Duarte, USA

    Nagarajan Vaidehi

  • , Department of Structural Biology, University of Pittsburgh School of Medic, Pittsburgh, USA

    Judith Klein-Seetharaman

Bibliographic Information

  • Book Title: Membrane Protein Structure and Dynamics

  • Book Subtitle: Methods and Protocols

  • Editors: Nagarajan Vaidehi, Judith Klein-Seetharaman

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-62703-023-6

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC 2012

  • Hardcover ISBN: 978-1-62703-022-9Published: 12 September 2012

  • Softcover ISBN: 978-1-4939-6233-4Published: 23 August 2016

  • eBook ISBN: 978-1-62703-023-6Published: 14 September 2012

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 357

  • Topics: Protein Science, Membrane Biology

Buy it now

Buying options

eBook USD 84.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 159.00
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 109.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access