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  • © 2012

Genomic Imprinting

Methods and Protocols

Editors:

  • Surveys cutting-edge technologies applied in the field
  • Features detailed and practical laboratory protocols
  • Includes expert tips on troubleshooting and avoiding pitfalls
  • Includes supplementary material: sn.pub/extras
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 925)

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Table of contents (19 protocols)

  1. Front Matter

    Pages i-xi
  2. Parent-of-Origin Effects

    1. Front Matter

      Pages 1-1
    2. Uniparental Embryos in the Study of Genomic Imprinting

      • Yong Cheng, Dasari Amarnath, Keith E. Latham
      Pages 3-19
    3. Derivation of Induced Pluripotent Stem Cells by Retroviral Gene Transduction in Mammalian Species

      • Masanori Imamura, Hironobu Okuno, Ikuo Tomioka, Yoshimi Kawamura, Zachary Yu-Ching Lin, Ryusuke Nakajima et al.
      Pages 21-48
    4. Generation of Trophoblast Stem Cells

      • Michael C. Golding
      Pages 49-59
    5. Immunomagnetic Purification of Murine Primordial Germ Cells

      • Emily Y. Smith, James L. Resnick
      Pages 61-66
  3. Identifying Imprinted Genes

    1. Front Matter

      Pages 67-67
    2. Data Mining as a Discovery Tool for Imprinted Genes

      • Chelsea Brideau, Paul Soloway
      Pages 89-134
  4. Identifying the Regulatory Features of Imprinted Domains

    1. Front Matter

      Pages 135-135
  5. Epigenetics of Imprinted Regions

    1. Front Matter

      Pages 147-147
    2. Methylated DNA Immunoprecipitation (MeDIP) from Low Amounts of Cells

      • Julie Borgel, Sylvain Guibert, Michael Weber
      Pages 149-158
    3. Quantitative Chromosome Conformation Capture

      • Raffaella Nativio, Yoko Ito, Adele Murrell
      Pages 173-185
  6. Analysis of Imprinted Expression

    1. Front Matter

      Pages 199-199

About this book

Genomic imprinting, the process by which the non-equivalence of the paternal and maternal genomes is established, has been fascinating us for over three decades and has provided many emerging scientists with the chance to hit their stride in a frontier posing many unexpected questions and even more surprising answers. In Genomic Imprinting: Methods and Protocols, experts in the field provide a survey of the technologies that are being applied to advance the study of imprinting.  This detailed volume features new technologies that are accelerating the pace of discovery of imprinted genes and characterization of their epigenetic profile, bioinformatic procedures for prediction and comparative analyses of imprinted genes, as well as methods in embryology and basic molecular biology that have been employed for many years, some appearing in new versions for small cell numbers.  Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 

Authoritative and easy to use, Genomic Imprinting: Methods and Protocols will aid scientists in  unveiling both much awaited answers and all-new questions to keep this vital field busy for many exciting years to come.

Editors and Affiliations

  • School of Medicine, Fels Institute, Temple University, Philadelphia, USA

    Nora Engel

Bibliographic Information

  • Book Title: Genomic Imprinting

  • Book Subtitle: Methods and Protocols

  • Editors: Nora Engel

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-62703-011-3

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC 2012

  • Hardcover ISBN: 978-1-62703-010-6Published: 21 August 2012

  • Softcover ISBN: 978-1-4939-5966-2Published: 23 August 2016

  • eBook ISBN: 978-1-62703-011-3Published: 21 August 2012

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 297

  • Topics: Human Genetics

Buy it now

Buying options

eBook USD 84.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 159.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 109.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access