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  • Book
  • © 2012

Helicobacter Species

Methods and Protocols

  • Presents methods and protocols commonly used to study Helicobacter
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 921)

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Softcover Book USD 119.99
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Hardcover Book USD 169.99
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Table of contents (26 protocols)

  1. Front Matter

    Pages i-x
  2. Introduction

    • Jean Marie Houghton
    Pages 1-5
  3. Helicobacter pylori: An Overview

    • Jennifer M. Noto, Richard M. Peek Jr.
    Pages 7-10
  4. The Helicobacter pylori cag Pathogenicity Island

    • Jennifer M. Noto, Richard M. Peek Jr.
    Pages 41-50
  5. Cell Culture-Based Assays to Test for Bacterial Adherence and Internalization

    • Deepa Raju, David Rizzuti, Nicola L. Jones
    Pages 69-76
  6. Cell Culture Assays to Evaluate Bacterial Toxicity and Virulence

    • Deepa Raju, David Rizzuti, Nicola L. Jones
    Pages 77-88
  7. Rodent Models of Helicobacter Infection, Inflammation, and Disease

    • Songhua Zhang, Steven F. Moss
    Pages 89-98
  8. Bacterial Culture and Inoculation of Mice (Simple Infection)

    • Brian M. Gray, Kathryn A. Eaton
    Pages 99-107
  9. Adoptive Transfer of Splenocytes to Immunocompromised Mice

    • Brian M. Gray, Kathryn A. Eaton
    Pages 109-112
  10. Isolation of Gastric Lamina Propria Leukocytes

    • Brian M. Gray, Kathryn A. Eaton
    Pages 113-116
  11. Delayed-Type Hypersensitivity Determination

    • Brian M. Gray, Kathryn A. Eaton
    Pages 117-118
  12. Verifying and Quantifying Helicobacter pylori Infection Status of Research Mice

    • Mark T. Whary, Zhongming Ge, James G. Fox
    Pages 143-156

About this book

Identifying  Helicobacter infection as the leading cause of peptic ulcer disease and gastric cancer has dramatically altered the treatment of these disease states.  Over the last several decades, scientists have come to understand that the interplay between the bacteria, the host, and the environment all contribute to the clinical outcome of infection. In Helicobacter Species: Methods and Protocols, expert researchers in the field detail many of the methods and which are now commonly used to study Helicobacter infection. These include protocols and methods that have evolved over time, and standards across the field have been established which are essential for optimal outcomes and to allow comparison of data across different laboratories. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

 

Authoritative and practical, Helicobacter Species: Methods and Protocols seeks to aid scientists in further study of this crucially important research into Helicobacter research.

Editors and Affiliations

  • Medical School, Dept. Medicine, University of Massachusetts, Worcester, USA

    JeanMarie Houghton

Bibliographic Information

  • Book Title: Helicobacter Species

  • Book Subtitle: Methods and Protocols

  • Editors: JeanMarie Houghton

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-62703-005-2

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC 2012

  • Hardcover ISBN: 978-1-62703-004-5Published: 27 September 2012

  • Softcover ISBN: 978-1-4939-5907-5Published: 23 August 2016

  • eBook ISBN: 978-1-62703-005-2Published: 27 September 2012

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: X, 258

  • Topics: Microbiology, Bacteriology

Buy it now

Buying options

eBook USD 89.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 119.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 169.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access