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Xenopus Protocols

Post-Genomic Approaches

  • Book
  • © 2012

Overview

Editors:
  1. STEFAN HOPPLER

    1. , Institute of Medical Sciences, University of Aberdeen, ABERDEEN, United Kingdom

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    You can also search for this editor in PubMed   Google Scholar

  2. Peter D Vize

    1. , Department of Biological Science, University of Calgary, Calgary, Canada

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    You can also search for this editor in PubMed   Google Scholar

  • Focuses on post-genomic approaches to the model system of Xenopus
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 917)

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Table of contents (31 protocols)

  1. Front Matter

    Pages i-xiii
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  2. Xenopus Tropicalis

    1. Front Matter

      Pages 1-1
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    2. Xenopus tropicalis as a Model Organism for Genetics and Genomics: Past, Present, and Future

      • Robert M. Grainger
      Pages 3-15

    3. Husbandry of Xenopus tropicalis

      • Alan Jafkins, Anita Abu-Daya, Anna Noble, Lyle B. Zimmerman, Matthew Guille
      Pages 17-31

    4. Generating Diploid Embryos from Xenopus tropicalis

      • Florencia del Viso, Mustafa Khokha
      Pages 33-41

    5. Navigating the Xenopus tropicalis Genome

      • Ira L. Blitz
      Pages 43-65

  3. Genetics in Xenopus

    1. Front Matter

      Pages 67-67
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    2. Genetic Analysis of Xenopus tropicalis

      • Timothy J. Geach, Derek L. Stemple, Lyle B. Zimmerman
      Pages 69-110

    3. Forward Genetic Screens in Xenopus Using Transposon-Mediated Insertional Mutagenesis

      • Donald A. Yergeau, Clair M. Kelley, Haiqing Zhu, Emin Kuliyev, Paul E. Mead
      Pages 111-127

    4. Targeted Gene Disruption with Engineered Zinc-Finger Nucleases (ZFNs)

      • John J. Young, Richard M. Harland
      Pages 129-141

    5. Reverse Genetic Studies Using Antisense Morpholino Oligonucleotides

      • Yanan Zhao, Shoko Ishibashi, Enrique Amaya
      Pages 143-154

    6. Chemical Genetics and Drug Discovery in Xenopus

      • Matthew L. Tomlinson, Adam E. Hendry, Grant N. Wheeler
      Pages 155-166

    7. Maternal mRNA Knock-down Studies: Antisense Experiments Using the Host-Transfer Technique in Xenopus laevis and Xenopus tropicalis

      • David J. Olson, Alissa M. Hulstrand, Douglas W. Houston
      Pages 167-182

  4. Xenopus Trangenesis

    1. Front Matter

      Pages 183-183
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    2. Generating Transgenic Frog Embryos by Restriction Enzyme Mediated Integration (REMI)

      • Shoko Ishibashi, Kristen L. Kroll, Enrique Amaya
      Pages 185-203

    3. A Simple Method of Transgenesis Using I-Sce I Meganuclease in Xenopus

      • Shoko Ishibashi, Nick R. Love, Enrique Amaya
      Pages 205-218

    4. Using ΦC31 Integrase to Mediate Insertion of DNA in Xenopus Embryos

      • You E. Li, Bryan G. Allen, Daniel L. Weeks
      Pages 219-230

    5. Xenopus Transgenics: Methods Using Transposons

      • Clair M. Kelley, Donald A. Yergeau, Haiqing Zhu, Emin Kuliyev, Paul E. Mead
      Pages 231-243

    6. Comparative Genomics-Based Identification and Analysis of Cis-Regulatory Elements

      • Hajime Ogino, Haruki Ochi, Chihiro Uchiyama, Sarah Louie, Robert M. Grainger
      Pages 245-263

    7. Tet-On Binary Systems for Tissue-Specific and Inducible Transgene Expression

      • Daniel R. Buchholz
      Pages 265-275
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Keywords

About this book

During the 20th century Xenopus has flourished as an accessible experimental model system for studying vertebrate biology. The second edition of  Xenopus Protocols:Post-Genomic Approaches expands upon the previous edition with novel approaches inspired by Xenopus tropicalis and the sequencing of its genome. Xenopus tropicalis genomics and tractable genetics combined with the unique experimental advantages of Xenopus laevis and their distinctive phylogenetic position make them unique model systems for vertebrate embryonic development in the 21st century. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

 

Authoritative and practical, Xenopus Protocols: Post-Genomic Approaches, Second Edition provides a toolkit that will enable every laboratory to maximize the power of this extraordinary experimental system in the post-genomic age.  

Editors and Affiliations

  • , Institute of Medical Sciences, University of Aberdeen, ABERDEEN, United Kingdom

    STEFAN HOPPLER

  • , Department of Biological Science, University of Calgary, Calgary, Canada

    Peter D Vize

Bibliographic Information

  • Book Title: Xenopus Protocols

  • Book Subtitle: Post-Genomic Approaches

  • Editors: STEFAN HOPPLER, Peter D Vize

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-61779-992-1

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC 2012

  • Hardcover ISBN: 978-1-61779-991-4Published: 06 September 2012

  • Softcover ISBN: 978-1-4939-5953-2Published: 23 August 2016

  • eBook ISBN: 978-1-61779-992-1Published: 06 September 2012

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 2

  • Number of Pages: XIII, 568

  • Topics: Human Genetics, Animal Genetics and Genomics, Animal Models

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