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Plant Salt Tolerance

Methods and Protocols

  • Book
  • © 2012

Overview

Editors:
  1. Sergey Shabala

    1. School of Agricultural Science, University of Tasmania, Hobart, Australia

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    You can also search for this editor in PubMed   Google Scholar

  2. Tracey Ann Cuin

    1. INRA-SupAgro, BPMP-IBIPGC, Montpellier, France

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 913)

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Table of contents (28 protocols)

  1. Front Matter

    Pages i-xiv
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  2. Microelectrode Techniques

    1. Front Matter

      Pages 1-1
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    2. Patch-Clamp Protocols to Study Cell Ionic Homeostasis Under Saline Conditions

      • Ana María Velarde-Buendía, René Alberto Enríquez-Figueroa, Igor Pottosin
      Pages 3-18

    3. Studying Plant Salt Tolerance with the Voltage Clamp Technique

      • Zhong-Hua Chen, Dezhi Wu, Cornelia Eisenach, Adrian Hills, Guoping Zhang, Michael R. Blatt
      Pages 19-33

    4. Using the Multifunctional Xylem Probe for in situ Studies of Plant Water and Ion Relations Under Saline Conditions

      • Lars H. Wegner
      Pages 35-66

    5. Measuring Intracellular Ion Concentrations with Multi-Barrelled Microelectrodes

      • Anthony J. Miller, Susan Smith
      Pages 67-77

    6. Single-Cell Sampling and Analysis (SiCSA)

      • Wieland Fricke
      Pages 79-100

    7. Non-invasive Flux Measurements Using Microsensors: Theory, Limitations, and Systems

      • Ian Newman, Shao-Liang Chen, D. Marshall Porterfield, Jian Sun
      Pages 101-117

    8. Quantifying Kinetics of Net Ion Fluxes from Plant Tissues by Non-invasive Microelectrode Measuring MIFE Technique

      • Sergey Shabala, Tracey Ann Cuin, Lana Shabala, Ian Newman
      Pages 119-134

  4. Biochemical Assays

    1. Front Matter

      Pages 201-201
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    2. Metabolomics for Salinity Research

      • Ute Roessner, Diane M. Beckles
      Pages 203-215

    3. Purification of Plant Plasma Membranes by Two-Phase Partitioning and Measurement of H+ Pumping

      • Anette Lund, Anja Thoe Fuglsang
      Pages 217-223

    4. Determination of Reactive Oxygen Species in Salt-Stressed Plant Tissues

      • Andrés Alberto Rodríguez, Edith L. Taleisnik
      Pages 225-236

    5. Quantification of the Antioxidant Activity in Salt-Stressed Tissues

      • Jelena J. DragiÅ¡ić Maksimović, Branka D. Živanović
      Pages 237-250
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Keywords

About this book

Soil salinity is destroying several hectares of arable land every minute. Because remedial land management cannot completely solve the problem, salt tolerant crops or plant species able to remove excessive salt from the soil could contribute significantly to managing the salinity problem. The key to engineering crops for salt tolerance lies in a thorough understanding of the physiological mechanisms underlying the adaptive responses of plants to salinity. Plant Salt Tolerance: Methods and Protocols describes recent advances and techniques employed by researchers to understand the molecular and ionic basis of salinity tolerance and to investigate the mechanisms of salt stress perception and signalling in plants. With chapters written by leading international scientists, this book covers nearly 30 different methods, such as microelectrode and molecular methods, imaging techniques, as well as various biochemical assays. Written in the highly successful Methods in Molecular Biologyâ„¢ series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.

 

Authoritative and easily accessible, Plant Salt Tolerance: Methods and Protocols serves as an essential read for every student or researcher tackling various aspects of the salinity problem.

Editors and Affiliations

  • School of Agricultural Science, University of Tasmania, Hobart, Australia

    Sergey Shabala

  • INRA-SupAgro, BPMP-IBIPGC, Montpellier, France

    Tracey Ann Cuin

Bibliographic Information

  • Book Title: Plant Salt Tolerance

  • Book Subtitle: Methods and Protocols

  • Editors: Sergey Shabala, Tracey Ann Cuin

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-61779-986-0

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC 2012

  • Hardcover ISBN: 978-1-61779-985-3Published: 16 August 2012

  • Softcover ISBN: 978-1-4939-5939-6Published: 23 August 2016

  • eBook ISBN: 978-1-61779-986-0Published: 16 August 2012

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XIV, 432

  • Number of Illustrations: 30 b/w illustrations, 26 illustrations in colour

  • Topics: Plant Sciences, Plant Genetics and Genomics

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