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  • © 2012

Embryo Culture

Methods and Protocols

  • Sets the stage for future research and laboratory application involving embryo culture
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 912)

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Table of contents (23 protocols)

  1. Front Matter

    Pages i-x
  2. Historical Background of Gamete and Embryo Culture

    • Jacques Cohen, Don Rieger
    Pages 1-18
  3. Utility of Animal Models for Human Embryo Culture: Nonhuman Primates

    • Calvin Simerly, Gerald Schatten
    Pages 39-59
  4. Media Composition: Salts and Osmolality

    • Jay M. Baltz
    Pages 61-80
  5. Media Composition: Energy Sources and Metabolism

    • Deirdre Zander-Fox, Michelle Lane
    Pages 81-96
  6. Media Composition: Amino Acids and Cellular Homeostasis

    • Franchesca D. Houghton
    Pages 97-106
  7. Media Composition: Antioxidants/Chelators and Cellular Function

    • Catherine M. H. Combelles, Margo L. Hennet
    Pages 129-159
  8. Media Composition: pH and Buffers

    • Jason E. Swain
    Pages 161-175
  9. Media Composition: Growth Factors

    • Aparna Hegde, Barry Behr
    Pages 177-198
  10. Culture Systems: Single Step

    • Ronit Machtinger, Catherine Racowsky
    Pages 199-209
  11. Culture Systems: Sequential

    • Patrick Quinn
    Pages 211-230
  12. Culture Systems: Embryo Co-Culture

    • Yves J. R. Ménézo, Edouard Servy, Anna Veiga, André Hazout, Kay Elder
    Pages 231-247
  13. Culture Systems: Low-Oxygen Culture

    • Borut Kovačič
    Pages 249-272
  14. Culture Systems: Embryo Density

    • Michael L. Reed
    Pages 273-312
  15. Culture Systems: Air Quality

    • Theodore Thomas
    Pages 313-324
  16. Culture Systems: Mineral Oil Overlay

    • Dean E. Morbeck, Phoebe H. Leonard
    Pages 325-331

About this book

Assisted reproductive technologies have had a profound impact on biomedical research through transgenic animals, food supply and production, as wells as genetic gain of domestic species, and treatment of human infertility. While significant advances in embryo culture have occurred over the last few decades. In Embryo Culture: Methods and Protocols, expert researchers in the field detail many of the methods which are now commonly used to study human embryo culture. These include emerging methods  and the impact of embryo culture on epigenetics and offspring health is presented to set the stage for future research and laboratory application involving embryo culture. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Embryo Culture: Methods and Protocols seeks to aid scientists in the further study of this crucially important field of embryology and assisted reproductive technologies.

Reviews

From the reviews:

“It has been a really pleasure to follow the presentation of all the topics related to the improvements occurring in human embryo culture as they are here presented. … The editors, and all of the authors, have to be acknowledged for this very well done book that will help researchers, clinicians and reproductive biologists … .” (CarloAlberto Redi, European Journal of Histochemistry, Vol. 57, 2013)

Editors and Affiliations

  • , OB/GYN, University of Michigan, Ann Arbor, USA

    Gary D. Smith

  • , Center for Reproductive Medicine, University of Michigan, Ann Arbor, USA

    Jason E. Swain

  • Fertility Center of San Antonio, San Antonio, USA

    Thomas B. Pool

Bibliographic Information

  • Book Title: Embryo Culture

  • Book Subtitle: Methods and Protocols

  • Editors: Gary D. Smith, Jason E. Swain, Thomas B. Pool

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-61779-971-6

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC 2012

  • Hardcover ISBN: 978-1-61779-970-9Published: 25 July 2012

  • Softcover ISBN: 978-1-4939-5920-4Published: 23 August 2016

  • eBook ISBN: 978-1-61779-971-6Published: 26 July 2012

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: X, 433

  • Number of Illustrations: 22 b/w illustrations, 18 illustrations in colour

  • Topics: Cell Biology, Embryology

Buy it now

Buying options

eBook USD 84.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 159.00
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 109.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access