Editors:
- Includes cutting-edge methods and protocols
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
- Includes supplementary material: sn.pub/extras
Part of the book series: Methods in Molecular Biology (MIMB, volume 903)
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Table of contents (29 protocols)
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Front Matter
About this book
Sexually transmitted infections (STI) continue to be a major cause of morbidity and mortality, both in developed industrial countries as well as in the developing world. Human immunodeficiency virus infections and the ensuing opportunistic infections are a major drain on the human and financial resources of many countries in the developing world and even with the availability of effective treatment the epidemic is not yet contained. Screening in developing worlds is difficult and there is a need for simple reliable cheap diagnostic methods that can be performed at the point of care, on the same day and by staff with limited training. Molecular biological methods are very attractive for the diagnosis of STI since a well defined range of pathogens is responsible for the infection. Diagnosis of Sexually Transmitted Diseases: Methods and Protocols strives to cover the full range of molecular testing for STI. Chapters cover a variety of topics such as aspects of DNA extraction from small volume samples or difficult tissues, simple, nested or multiplex PCR, use of duplex primers or other modifications of primers and PCR conditions, sequence analysis for genotyping, denaturing gel analysis, microarrays using liquid beads or microspheres and silicon nanoparticle-enhanced microcantilever detection of DNA. Written in the successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.
Authoritative and easily accessible, Diagnosis of Sexually Transmitted Diseases: Methods and Protocols will be a valuable reference for the scientist looking to establish molecular methodologies for the diagnosis of STI tests intheir own lab.
Reviews
From the book reviews:
“This volume of Methods in Molecular Biology is the first to provide a comprehensive overview of molecular methods for sexually transmitted infections (STI). … This is a unique addition to the field of STI diagnostics and will be a useful reference for laboratory scientists wishing to incorporate the methods into their laboratories as well as for researchers and clinicians wishing to enhance their understanding of this area.” (Ameeta E. Singh, Doody’s Book Reviews, February, 2013)Editors and Affiliations
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University Hospital, Institute of Medical Microbiology, Heinrich-Heine-University, Düsseldorf, Germany
Colin R. MacKenzie, Birgit Henrich
Bibliographic Information
Book Title: Diagnosis of Sexually Transmitted Diseases
Book Subtitle: Methods and Protocols
Editors: Colin R. MacKenzie, Birgit Henrich
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-61779-937-2
Publisher: Humana Totowa, NJ
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media New York 2012
Hardcover ISBN: 978-1-61779-936-5Published: 12 July 2012
Softcover ISBN: 978-1-4939-5885-6Published: 23 August 2016
eBook ISBN: 978-1-61779-937-2Published: 11 July 2012
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: XIV, 439
Topics: Infectious Diseases, Virology