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Vaccinia Virus and Poxvirology

Methods and Protocols

  • Book
  • © 2012

Overview

Editors:
  1. Stuart N. Isaacs

    1. School of Medicine and the Philadelphia, Division of Infectious Disease, University of Pennsylvania, Philadelphia, USA

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  • Details the most current poxvirus protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 890)

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Table of contents (18 protocols)

  1. Front Matter

    Pages i-xiii
    Download chapter PDF

  2. Working Safely with Vaccinia Virus: Laboratory Technique and Review of Published Cases of Accidental Laboratory Infections

    • Stuart N. Isaacs
    Pages 1-22

  3. In-Fusion® Cloning with Vaccinia Virus DNA Polymerase

    • Chad R. Irwin, Andrew Farmer, David O. Willer, David H. Evans
    Pages 23-35

  4. Genetic Manipulation of Poxviruses Using Bacterial Artificial Chromosome Recombineering

    • Matthew G. Cottingham
    Pages 37-57

  5. Easy and Efficient Protocols for Working with Recombinant Vaccinia Virus MVA

    • Melanie Kremer, Asisa Volz, Joost H. C. M. Kreijtz, Robert Fux, Michael H. Lehmann, Gerd Sutter
    Pages 59-92

  6. Isolation of Recombinant MVA Using F13L Selection

    • Juana M. Sánchez-Puig, María M. Lorenzo, Rafael Blasco
    Pages 93-111

  7. Screening for Vaccinia Virus Egress Inhibitors: Separation of IMV, IEV, and EEV

    • Chelsea M. Byrd, Dennis E. Hruby
    Pages 113-121

  8. Imaging of Vaccinia Virus Entry into HeLa Cells

    • Cheng-Yen Huang, Wen Chang
    Pages 123-133

  9. New Method for the Assessment of Molluscum Contagiosum Virus Infectivity

    • Subuhi Sherwani, Niamh Blythe, Laura Farleigh, Joachim J. Bugert
    Pages 135-146

  10. An Intradermal Model for Vaccinia Virus Pathogenesis in Mice

    • Leon C. W. Lin, Stewart A. Smith, David C. Tscharke
    Pages 147-159

  11. Measurements of Vaccinia Virus Dissemination Using Whole Body Imaging: Approaches for Predicting of Lethality in Challenge Models and Testing of Vaccines and Antiviral Treatments

    • Marina Zaitseva, Senta Kapnick, Hana Golding
    Pages 161-176

  12. Mousepox, A Small Animal Model of Smallpox

    • David Esteban, Scott Parker, Jill Schriewer, Hollyce Hartzler, R. Mark Buller
    Pages 177-198

  13. Analyzing CD8 T Cells in Mouse Models of Poxvirus Infection

    • Inge E. A. Flesch, Yik Chun Wong, David C. Tscharke
    Pages 199-218

  14. Generation and Characterization of Monoclonal Antibodies Specific for Vaccinia Virus

    • Xiangzhi Meng, Yan Xiang
    Pages 219-232

  15. Bioinformatics for Analysis of Poxvirus Genomes

    • Melissa Da Silva, Chris Upton
    Pages 233-258

  16. Antigen Presentation Assays to Investigate Uncharacterized Immunoregulatory Genes

    • Rachel L. Roper
    Pages 259-271

  17. Characterization of Poxvirus-Encoded Proteins that Regulate Innate Immune Signaling Pathways

    • Florentina Rus, Kayla Morlock, Neal Silverman, Ngoc Pham, Girish J. Kotwal, William L. Marshall
    Pages 273-288

  18. Application of Quartz Crystal Microbalance with Dissipation Monitoring Technology for Studying Interactions of Poxviral Proteins with Their Ligands

    • Amod P. Kulkarni, Lauriston A. Kellaway, Girish J. Kotwal
    Pages 289-303

  19. Central Nervous System Distribution of the Poxviral Proteins After Intranasal Administration of Proteins and Titering of Vaccinia Virus in the Brain After Intracranial Administration

    • Amod P. Kulkarni, Dhirendra Govender, Lauriston A. Kellaway, Girish J. Kotwal
    Pages 305-326

  20. Back Matter

    Pages 327-332
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Keywords

About this book

Since the first edition of Vaccinia Virus and Poxvirology: Methods and Protocols was published, a number of important events related to poxvirology have occurred, such as FDA approval of a culture-based live smallpox vaccine and the vaccination of large numbers of U.S. military and relatively large numbers of U.S. civilians. Novel anti-poxvirus therapeutics have been developed and have been used in emergency settings. The second edition of Vaccinia Virus and Poxvirology expands upon the previous edition with entirely new sets of protocols. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

 Authoritative and practical, Vaccinia Virus and Poxvirology: Methods and Protocols, Second Edition seeks to aid scientists in continuing to study poxviruses using new tools and approaches.

Editors and Affiliations

  • School of Medicine and the Philadelphia, Division of Infectious Disease, University of Pennsylvania, Philadelphia, USA

    Stuart N. Isaacs

Bibliographic Information

  • Book Title: Vaccinia Virus and Poxvirology

  • Book Subtitle: Methods and Protocols

  • Editors: Stuart N. Isaacs

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-61779-876-4

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC 2012

  • Hardcover ISBN: 978-1-61779-875-7Published: 12 June 2012

  • Softcover ISBN: 978-1-4939-6083-5Published: 23 August 2016

  • eBook ISBN: 978-1-61779-876-4Published: 12 June 2012

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 2

  • Number of Pages: XIII, 332

  • Number of Illustrations: 55 b/w illustrations, 13 illustrations in colour

  • Topics: Infectious Diseases, Virology

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