Editors:
- Aims at applying gene therapy during pregnancy for the prevention of human disease
- Provides step-by-step detail essential fro reproducible results
- Contains key notes and implementation advice from the experts
- Includes supplementary material: sn.pub/extras
Part of the book series: Methods in Molecular Biology (MIMB, volume 891)
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Table of contents (17 protocols)
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Front Matter
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Back Matter
About this book
The emerging field of prenatal gene therapy is founded on scientific and technical advances in fetal medicine, molecular biology and gene therapy. This preclinical research subject aims at applying gene therapy during pregnancy for the prevention of human diseases caused by early onset congenital or gestation related conditions.  In Prenatal Gene Therapy: Concepts, Methods and Protocols, expert researchers in the field detail many of the protocols which are now commonly used to study gene therapy, fetal medicine and medical ethics. These include detailed protocols for vector production, for breeding and husbandry of the animal models, for the surgical procedures of gene delivery in large and small animals and for the methods of gene transfer analysis. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.
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Thorough and intuitive, Prenatal Gene Therapy: Concepts, Methods and Protocols seeks to aid scientists in the further study of prenatal disease and gene therapy projects beyond the scope of fetal medicine.
Editors and Affiliations
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National Heart & Lung Inst., Molecular & Cellular Medicine Sec., Imperial College London, London, United Kingdom
Charles Coutelle
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Reader in Gene Transfer Technology, Gene Transfer Technology Group, University College London, London, United Kingdom
Simon N. Waddington
Bibliographic Information
Book Title: Prenatal Gene Therapy
Book Subtitle: Concepts, Methods, and Protocols
Editors: Charles Coutelle, Simon N. Waddington
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-61779-873-3
Publisher: Humana Totowa, NJ
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media, LLC. 2012
Hardcover ISBN: 978-1-61779-872-6
Softcover ISBN: 978-1-4939-5911-2
eBook ISBN: 978-1-61779-873-3
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: X, 392
Number of Illustrations: 26 b/w illustrations, 7 illustrations in colour
Topics: Animal Genetics and Genomics, Human Genetics, Gene Therapy