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Retinal Development

Methods and Protocols

  • Book
  • © 2012

Overview

Editors:
  1. Shu-Zhen Wang

    1. University of Alabama at Birmingham, Birmingham, USA

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  • Details methodolgies that can be modified and applied to the studies of rential development
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 884)

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Table of contents (25 protocols)

  1. Front Matter

    Pages i-xiv
    Download chapter PDF

  2. Manipulation of Gene Expression In Vivo

    1. Front Matter

      Pages 1-1
      Download chapter PDF

  3. Manipulation of Gene Expression in vivo

    1. Conditional Control of Gene Expression in the Mouse Retina

      • Qian Ding, Lin Gan
      Pages 3-15

    2. Generation of Transgenic Xenopus Using Restriction Enzyme-Mediated Integration

      • Mohammad Haeri, Barry E. Knox
      Pages 17-39

    3. In Vivo Functional Analysis of Transcription Factor: Response Element Interaction Using Transgenic Xenopus laevis

      • Heithem M. El-Hodiri, Yi Pan, Lisa E. Kelly
      Pages 41-51

    4. Subretinal Delivery and Electroporation in Pigmented and Nonpigmented Adult Mouse Eyes

      • John M. Nickerson, Penny Goodman, Micah A. Chrenek, Christiana J. Bernal, Lennart Berglin, T. Michael Redmond et al.
      Pages 53-69

    5. In Ovo Eye Electroporation

      • Teri L. Belecky-Adams, Scott R. Hudson, Sarika Tiwari
      Pages 71-89

    6. Targeted Microinjection of Synthetic mRNAs to Alter Retina Gene Expression in Xenopus Embryos

      • Sally A. Moody
      Pages 91-111

  5. In Vitro Systems

    1. Front Matter

      Pages 153-153
      Download chapter PDF

  6. In vitro Systems

    1. In Vitro Explant Culture and Related Protocols for the Study of Mouse Retinal Development

      • Kangxin Jin, Mengqing Xiang
      Pages 155-165

    2. In Vitro Biochemical Assays to Monitor Rhodopsin Function

      • Joshua Sammons, Alecia K. Gross
      Pages 167-181

    3. Transfection of Primary Embryonic Chicken Retinal Cells Using Cationic Lipid

      • Yi-Wen Hsieh, Xian-Jie Yang
      Pages 183-192

    4. Production of High-Titer RCAS Retrovirus

      • Run-Tao Yan, Shu-Zhen Wang
      Pages 193-199

    5. Chick Retinal Pigment Epithelium Transdifferentiation Assay for Proneural Activities

      • Shu-Zhen Wang, Run-Tao Yan
      Pages 201-209

  7. Regeneration/Stem Cells

    1. Front Matter

      Pages 211-211
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    2. Studying the Generation of Regenerated Retinal Neuron from Müller Glia in the Mouse Eye

      • Mike O. Karl, Thomas A. Reh
      Pages 213-227
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Keywords

About this book

In recent years, there have been major advances in the concepts and methodologies used in the study of retinal development at both cellular and molecular levels. These advanced methodologies have allowed and will continue to allow researchers to gain new insights into the molecular mechanisms underlying retinal development. In Retinal Development: Methods and Protocols, expert researchers in the field detail many of the protocols  used for a wide range of experiments. These include protocols  and techniques for manipulating gene expression in vivo, tracing cell fates with modernized classic blastomere manipulation in Xenopus and with Cre-based technique in mouse and in zebrafish, retinal regeneration and stem cell-based replacement, and ERG (function) recording and non-invasive imaging. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

 

Authoritative and practical, Retinal Development: Methods and Protocols provides methodologies crucial to the success of increasingly more complex and often challenging investigations in the fields of retinal development and other biological and biomedical research. 

Editors and Affiliations

  • University of Alabama at Birmingham, Birmingham, USA

    Shu-Zhen Wang

Bibliographic Information

  • Book Title: Retinal Development

  • Book Subtitle: Methods and Protocols

  • Editors: Shu-Zhen Wang

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-61779-848-1

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC 2012

  • Hardcover ISBN: 978-1-61779-847-4Published: 12 June 2012

  • Softcover ISBN: 978-1-4939-6252-5Published: 04 May 2017

  • eBook ISBN: 978-1-61779-848-1Published: 12 June 2012

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XIV, 367

  • Number of Illustrations: 58 b/w illustrations, 21 illustrations in colour

  • Topics: Molecular Medicine, Developmental Biology

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