Editors:
- Provides step-by-step laboratory protocols, from conventional methods to state-of-the-art high throughput approaches and even computational data processing techniques
- Contains expert tips on troubleshooting known pitfalls
- Lists the necessary materials and provides the needed background information for all experiments
Part of the book series: Methods in Molecular Biology (MIMB, volume 883)
Buy it now
Buying options
Tax calculation will be finalised at checkout
Other ways to access
This is a preview of subscription content, log in via an institution to check for access.
Table of contents (17 protocols)
-
Front Matter
-
Back Matter
About this book
RNA abundance analysis is one of the most important approaches for gene expression studies in the field of molecular biology. In RNA Abundance Analysis: Methods and Protocols, expert researchers cover a wide range of techniques on RNA extraction, detection, quantification, visualization, and genome-wide profiling, from conventional methods to state-of-the-art high throughput approaches. This volume includes detailed techniques to examine mRNAs, small non-coding RNAs, protein-associated small RNAs, sulfur-containing RNAs, viral and satellite RNAs, RNA isoforms, and alternatively spliced RNA variants from various organisms, as well as key discussions of computational data processing for genome-wide datasets. Written for the highly successful Methods in Molecular Biology™ series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Â
Essential and easy to use, RNA Abundance Analysis: Methods and Protocols provides a comprehensive set of techniques and methods on isolating and analyzing mRNAs, small RNAs, and modified RNAs, which can assist you in your gene expression studies.Editors and Affiliations
-
, Dept. Plant Pathology & Microbiology, University of California, Riverside, USA
Hailing Jin
-
, Division of Plant Sciences, University of Missouri, Columbia, USA
Walter Gassmann
Bibliographic Information
Book Title: RNA Abundance Analysis
Book Subtitle: Methods and Protocols
Editors: Hailing Jin, Walter Gassmann
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-61779-839-9
Publisher: Humana Totowa, NJ
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media, LLC 2012
Hardcover ISBN: 978-1-61779-838-2Published: 21 May 2012
Softcover ISBN: 978-1-4939-5916-7Published: 23 August 2016
eBook ISBN: 978-1-61779-839-9Published: 16 May 2012
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: XIII, 231
Number of Illustrations: 22 b/w illustrations
Topics: Human Genetics, Gene Expression