Editors:
- Features the important effects of S1P in cell development and physiopathology
- Includes detailed, tried-and-true protocols, ready for the lab
- Contains expert tips and key troubleshooting advice to ensure successful results
- Includes supplementary material: sn.pub/extras
Part of the book series: Methods in Molecular Biology (MIMB, volume 874)
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Table of contents (16 protocols)
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Front Matter
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Back Matter
About this book
Sphingosine-1-phosphate is a bioactive lysophospholipid which has become, in recent years, the focus of much research interest as it has widespread developmental and physio-pathological actions, controlling events within the nervous, reproductive, gastrointestinal, vascular, respiratory, and immune systems, in addition to having a prominent role in cancer, early mammalian embryogenesis, and stem cells. In Sphingosine-1-Phosphate: Methods and Protocols, worldwide experts in the S1P field describe in-depth techniques in an array of cell types and with various physiological applications, showcasing the important effects of S1P in development and in physiopathology. As a volume in the Methods in Molecular Biology™ series, chapters contain introductions to their respective topics, lists of the relevant materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips for troubleshooting and avoiding known pitfalls.
Authoritative and timely, Sphingosine-1-Phosphate: Methods and Protocols is a key resource for scientists working in this expanding and dynamic field.
Editors and Affiliations
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University of Melbourne, Parkville, Australia
Alice Pébay
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Sprott Centre for Stem Cell Research, Regenerative Medicine Program, Ottawa Hospital Research Institute, Ottawa, Canada
Kursad Turksen
Bibliographic Information
Book Title: Sphingosine-1-Phosphate
Book Subtitle: Methods and Protocols
Editors: Alice Pébay, Kursad Turksen
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-61779-800-9
Publisher: Humana Totowa, NJ
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media, LLC 2012
Hardcover ISBN: 978-1-61779-799-6Published: 19 April 2012
Softcover ISBN: 978-1-4939-5963-1Published: 23 August 2016
eBook ISBN: 978-1-61779-800-9Published: 23 April 2012
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: XI, 221
Number of Illustrations: 29 b/w illustrations, 10 illustrations in colour
Topics: Cell Biology, Cell Culture