In Vivo Cellular Imaging Using Fluorescent Proteins

1. Front Matter

   Pages i-xiii

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2. Assessing Cancer Cell Migration and Metastatic Growth In Vivo in the Chick Embryo Using Fluorescence Intravital Imaging

   • Hon Sing Leong, Ann F. Chambers, John D. Lewis

   Pages 1-14

3. The Use of Fluorescent Proteins for Intravital Imaging of Cancer Cell Invasion

   • James Hulit, Dmitriy Kedrin, Bojana Gligorijevic, David Entenberg, Jeffrey Wyckoff, John Condeelis et al.

   Pages 15-30

4. High-Resolution In Vivo Imaging of Fluorescent Proteins Using Window Chamber Models

   • Gregory M. Palmer, Andrew N. Fontanella, Siqing Shan, Mark W. Dewhirst

   Pages 31-50

5. In Vivo Imaging of Pancreatic Cancer with Fluorescent Proteins in Mouse Models

   • Michael Bouvet, Robert M. Hoffman

   Pages 51-67

6. Lentivirus-Based DsRed-2-Transfected Pancreatic Cancer Cells for Deep In Vivo Imaging of Metastatic Disease

   • Zeqian Yu, Jiahua Zhou, Robert M. Hoffman

   Pages 69-83

7. Noninvasive and Real-Time Fluorescence Imaging of Peritoneal Metastasis in Nude Mice

   • Hayao Nakanishi, Seiji Ito, Makoto Matsui, Yuichi Ito, Kazunari Misawa, Yasuhiro Kodera

   Pages 85-95

8. Three-Dimensional In Vivo Imaging of Tumors Expressing Red Fluorescent Proteins

   • Alexander P. Savitsky, Irina G. Meerovich, Victoria V. Zherdeva, Lyaysan R. Arslanbaeva, Olga S. Burova, Darina V. Sokolova et al.

   Pages 97-114

9. Real-Time Visualization and Characterization of Tumor Angiogenesis and Vascular Response to Anticancer Therapies

   • H. Rosie Xing, Qingbei Zhang

   Pages 115-127

10. In Vivo Imaging of Human Cancer with Telomerase-Specific Replication-Selective Adenovirus

    • Toshiyoshi Fujiwara

    Pages 129-139

11. Real-Time Fluorescence Imaging of Abdominal, Pleural, and Lymphatic Metastases

    • Susanne Carpenter, Yuman Fong

    Pages 141-157

12. Real-Time Imaging of Tumors Using Replication-Competent Light-Emitting Microorganisms

    • Yong A. Yu, Stephanie Weibel, Aladar A. Szalay

    Pages 159-175

13. GFP-Transgenic Animals for In Vivo Imaging: Rats, Rabbits, and Pigs

    • Takashi Murakami, Eiji Kobayashi

    Pages 177-189

14. The Use of Fluorescent Proteins for Developing Cancer-Specific Target Imaging Probes

    • Thomas E. McCann, Nobuyuki Kosaka, Peter L. Choyke, Hisataka Kobayashi

    Pages 191-204

15. In Vivo Imaging of the Developing Mouse Embryonic Vasculature

    • Irina V. Larina, Mary E. Dickinson

    Pages 205-215

16. Screening Reef Corals for Novel GFP-Type Fluorescent Proteins by Confocal Imaging

    • Anya Salih

    Pages 217-233

17. What Does It Take to Improve Existing Fluorescent Proteins for In Vivo Imaging Applications?

    • Marc Zimmer

    Pages 235-241

18. In Vivo Imaging of Oligonucleotide Delivery

    • Fumitaka Takeshita, Ryou-u Takahashi, Jun Onodera, Takahiro Ochiya

    Pages 243-253

19. Subcellular Imaging In Vivo: The Next GFP Revolution

    • Robert M. Hoffman

    Pages 255-263

20. Back Matter

    Pages 265-269

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The discovery and genetic engineering of fluorescent proteins has revolutionized cell biology.  What was previously invisible in the cell often can be made visible with the use of fluorescent proteins. In Vivo Cellular Imaging Using Fluorescent Proteins: Methods and Protocols presents state-of-the-art research that has contributed to the fluorescent protein revolution to visualize biological processes in the live animal. This volume covers an array of topics from the employment of the chick CAM model using fluorescent proteins and other fluorescent probes, to intravital fluorescent imaging, as well as 3-dimensional imaging, and design instructions on how to create new and improved far-red and infrared fluorescent proteins, to name a few. Written in the successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.

 

Authoritative and easily accessible, In Vivo Cellular Imaging Using Fluorescent Proteins: Methods and Protocols is the first volume in the new field of in vivo cell biology and it serves both professionals and novices with its well-honed methodologies.

• 3-dimensional in vivo imaging
• intravital imaging
• red fluorescent proteins
• tumor angiogenesis
• tumor cell invasion

From the reviews:

“Robert M. Hoffman introduces In vivo Cellular Imaging Using Fluorescent proteins, the eighteen chapters book dedicated to the description of how fluorescence proteins have changed the way to analyze cellular processes in vivo. … This book is very well structured with several colored figures … . this is a useful book not only for those who study the biology of different cancer cells, but also for those who are interested in following the in vivo dynamic of cellular processes.” (Manuela Monti, European Journal of Histochemistry, Vol. 56, 2012)

• AntiCancer, Inc., San Diego, USA

  Robert M. Hoffman

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