Overview
- Examines the process of preparation of expression vectors and disruption of yeast cells to enable to isolation of the recombinant protein
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
- Includes supplementary material: sn.pub/extras
Part of the book series: Methods in Molecular Biology (MIMB, volume 866)
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Table of contents (20 protocols)
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Front Matter
Keywords
About this book
In the last few years, significant advances have been made in understanding how a yeast cell responds to the stress of producing a recombinant protein, and how this information can be used to engineer improved host strains. The molecular biology of the expression vector, through the choice of promoter, tag and codon optimization of the target gene, is also a key determinant of a high-yielding protein production experiment. Recombinant Protein Production in Yeast: Methods and Protocols examines the process of preparation of expression vectors, transformation to generate high-yielding clones, optimization of experimental conditions to maximize yields, scale-up to bioreactor formats and disruption of yeast cells to enable the isolation of the recombinant protein prior to purification. Written in the highly successful Methods in Molecular Biologyâ„¢ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.
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Authoritative and practical, Recombinant Protein Production in Yeast: Methods and Protocols, seeks to aid scientists in adopting yeast as a protein production host.
Editors and Affiliations
Bibliographic Information
Book Title: Recombinant Protein Production in Yeast
Book Subtitle: Methods and Protocols
Editors: Roslyn M. Bill
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-61779-770-5
Publisher: Humana Totowa, NJ
eBook Packages: Springer Protocols
Copyright Information: Springer Science+business Media, LLC 2012
Hardcover ISBN: 978-1-61779-769-9Published: 28 March 2012
Softcover ISBN: 978-1-4939-5871-9Published: 23 August 2016
eBook ISBN: 978-1-61779-770-5Published: 28 March 2012
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: XII, 248
Number of Illustrations: 41 b/w illustrations, 6 illustrations in colour
Topics: Human Genetics