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Plant DNA Fingerprinting and Barcoding

Methods and Protocols

  • Book
  • © 2012

Overview

Editors:
  1. Nikolaus J. Sucher

    1. , Department of S.T.E.M., Roxbury Community College, Roxbury Crossing, USA

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  2. James R. Hennell

    1. Centre for Complementary Medicine, Research, University of Western Sydney, Campbelltown, Australia

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    You can also search for this editor in PubMed   Google Scholar

  3. Maria C. Carles

    1. , Department of Natural Sciences, Northern Essex Community College, Haverhill, USA

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    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 862)

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Table of contents (15 protocols)

  1. Front Matter

    Pages i-x
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  2. A Taxonomist’s View on Genomic Authentication

    • Bernard R. Baum
    Pages 1-12

  3. DNA Fingerprinting, DNA Barcoding, and Next Generation Sequencing Technology in Plants

    • Nikolaus J. Sucher, James R. Hennell, Maria C. Carles
    Pages 13-22

  4. Challenges in the DNA Barcoding of Plant Material

    • Robyn S. Cowan, Michael F. Fay
    Pages 23-33

  5. Plant Genetics for Forensic Applications

    • David N. Zaya, Mary V. Ashley
    Pages 35-52

  6. DNA Purification from Multiple Sources in Plant Research with Homemade Silica Resins

    • Jian-Feng Li, Jen Sheen
    Pages 53-59

  7. Random Amplified Marker Technique for Plants Rich in Polyphenols

    • Tripta Jhang, Ajit Kumar Shasany
    Pages 61-74

  8. Amplified Fragment Length Polymorphism: An Invaluable Fingerprinting Technique for Genomic, Transcriptomic, and Epigenetic Studies

    • Ovidiu Paun, Peter Schönswetter
    Pages 75-87

  9. Restriction Fragment Length Polymorphism of the 5S-rRNA-NTS Region: A Rapid and Precise Method for Plant Identification

    • Cinzia Margherita Bertea, Giorgio Gnavi
    Pages 89-101

  10. ISSR: A Reliable and Cost-Effective Technique for Detection of DNA Polymorphism

    • Maryam Sarwat
    Pages 103-121

  11. Development of Sequence Characterized Amplified Region from Random Amplified Polymorphic DNA Amplicons

    • Kalpana Joshi, Preeti Chavan
    Pages 123-134

  12. Authentication of Medicinal Plants by SNP-Based Multiplex PCR

    • Ok Ran Lee, Min-Kyeoung Kim, Deok-Chun Yang
    Pages 135-147

  13. Multiplex PCR Method to Discriminate Artemisia iwayomogi from Other Artemisia Plants

    • Eui Jeong Doh, Seung-Eun Oh
    Pages 149-160

  14. Loop-Mediated Isothermal Amplification for the Detection of Plant Pathogens

    • Lisa I. Ward, Scott J. Harper
    Pages 161-170

  15. Genomic DNA Extraction and Barcoding of Endophytic Fungi

    • Patricia L. Diaz, James R. Hennell, Nikolaus J. Sucher
    Pages 171-179

  16. Using GenBank® for Genomic Authentication: A Tutorial

    • James R. Hennell, Paul M. D’Agostino, Samiuela Lee, Cheang S. Khoo, Nikolaus J. Sucher
    Pages 181-200

  17. Back Matter

    Pages 201-202
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Keywords

About this book

Molecular cloning and DNA-based analysis have become part of every molecular life science laboratory. The rapid adoption of DNA-based techniques has been facilitated by the introduction of the polymerase chain reaction (PCR), which has made cloning and characterization of DNA quick and relatively simple. PCR is virtually part of every variation of the plethora of approaches used for DNA fingerprinting today. Plant DNA Fingerprinting: Methods and Protocols aims to bring together the different currently available genome-based techniques into one repository. This volume contains detailed protocols for the preparation of plant genomic DNA, fingerprinting of plants for the detection of intra-species variations, the use of DNA barcoding, as well as methods for the bioinformatic analysis of data. Also included are several discussions on the broader issues of genome-based approaches in order to provide a sound understanding of the principles of these methods. Written in the successful Methods in Molecular Biologyâ„¢ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.

 

Authoritative and easily accessible, Plant DNA Fingerprinting: Methods and Protocols is tailored principally for those who seek to augment their current methods of plant analysis and quality control using genome-based approaches as well as for scientists and researchers in different plant sciences.

Editors and Affiliations

  • , Department of S.T.E.M., Roxbury Community College, Roxbury Crossing, USA

    Nikolaus J. Sucher

  • Centre for Complementary Medicine, Research, University of Western Sydney, Campbelltown, Australia

    James R. Hennell

  • , Department of Natural Sciences, Northern Essex Community College, Haverhill, USA

    Maria C. Carles

Bibliographic Information

  • Book Title: Plant DNA Fingerprinting and Barcoding

  • Book Subtitle: Methods and Protocols

  • Editors: Nikolaus J. Sucher, James R. Hennell, Maria C. Carles

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-61779-609-8

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC 2012

  • Hardcover ISBN: 978-1-61779-608-1Published: 15 March 2012

  • Softcover ISBN: 978-1-4939-6225-9Published: 23 August 2016

  • eBook ISBN: 978-1-61779-609-8Published: 14 March 2012

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: X, 202

  • Topics: Plant Sciences, Plant Genetics and Genomics

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