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  • © 2012

Plant Metabolomics

Methods and Protocols

  • Presents methods for all stages of the plant metabolomics workflow
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 860)

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Table of contents (18 protocols)

  1. Front Matter

    Pages i-xiii
  2. Practical Applications of Metabolomics in Plant Biology

    • Robert D. Hall, Nigel W. Hardy
    Pages 1-10
  3. Material Preparation

    1. Front Matter

      Pages 11-11
    2. Separating the Inseparable: The Metabolomic Analysis of Plant–Pathogen Interactions

      • J. William Allwood, Jim Heald, Amanda J. Lloyd, Royston Goodacre, Luis A. J. Mur
      Pages 31-49
    3. Precautions for Harvest, Sampling, Storage, and Transport of Crop Plant Metabolomics Samples

      • Benoît Biais, Stéphane Bernillon, Catherine Deborde, Cécile Cabasson, Dominique Rolin, Yaakov Tadmor et al.
      Pages 51-63
    4. Tissue Preparation Using Arabidopsis

      • Aimee M. Llewellyn, Jennie Lewis, Sonia J. Miller, Delia-Irina Corol, Michael H. Beale, Jane L. Ward
      Pages 65-81
  4. Chemical Analysis Approaches

    1. Front Matter

      Pages 83-83
    2. Solid Phase Micro-Extraction GC–MS Analysis of Natural Volatile Components in Melon and Rice

      • Harrie A. Verhoeven, Harry Jonker, Ric C. H. De Vos, Robert D. Hall
      Pages 85-99
    3. Profiling Primary Metabolites of Tomato Fruit with Gas Chromatography/Mass Spectrometry

      • Sonia Osorio, Phuc Thi Do, Alisdair R. Fernie
      Pages 101-109
    4. UPLC-MS-Based Metabolite Analysis in Tomato

      • Ilana Rogachev, Asaph Aharoni
      Pages 129-144
    5. High Precision Measurement and Fragmentation Analysis for Metabolite Identification

      • Madalina Oppermann, Nicolaie Eugen Damoc, Catharina Crone, Thomas Moehring, Helmut Muenster, Martin Hornshaw
      Pages 145-156
    6. Fourier Transform Ion Cyclotron Resonance Mass Spectrometry for Plant Metabolite Profiling and Metabolite Identification

      • J. William Allwood, David Parker, Manfred Beckmann, John Draper, Royston Goodacre
      Pages 157-176
    7. Combined NMR and Flow Injection ESI-MS for Brassicaceae Metabolomics

      • John M. Baker, Jane L. Ward, Michael H. Beale
      Pages 177-191
    8. ICP-MS and LC-ICP-MS for Analysis of Trace Element Content and Speciation in Cereal Grains

      • D. P. Persson, T. H. Hansen, K. H. Laursen, S. Husted, J. K. Schjoerring
      Pages 193-211
    9. The Use of Genomics and Metabolomics Methods to Quantify Fungal Endosymbionts and Alkaloids in Grasses

      • Susanne Rasmussen, Geoffrey A. Lane, Wade Mace, Anthony J. Parsons, Karl Fraser, Hong Xue
      Pages 213-226
  5. Data Analysis

    1. Front Matter

      Pages 227-227

About this book

Estimation of the metabolite complement of plant material involves a wide range of techniques and technologies and that breadth continues to increase.  Metabolomics research typically involves multiple sites for material preparation and analysis and most investigations are “high throughput”, meaning that chemical analysis of sample sets are inevitably carried out over an extended period of time.  In, Plant Metabolomics: Methods and Protocols expert researchers in the field detail many of the stages which are now commonly used to study plant metabolomics workflow. Stages of this workflow, up to and including the statistical analysis, accurate and detailed collection of meta-data are also essential for good process management, to satisfy reporting requirements and to ensure wider interpretability and reuse  results.Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

 

 

Through and intuitive Plant Metabolomics: Methods and Protocols,  seeks to aid scientists in the further study of the methods for all the stages of the plant metabolomics workflow.

Reviews

From the reviews:

“The chapters presented in this volume stay true to the Methods and Protocols series, with a short piece of background information, a highly detailed step-by-step protocol, and additional practical information that will allow beginners in the field to set up repeatable, high-quality metabolomics experiments. … this is an excellent addition to the series and can provide muchneeded support, especially to scientists starting in the growing field of plant metabolomics.” (Alexandre Foito, Annals of Botany, Vol. 113 (7), April, 2014)

“The book ‘Plant Metabolomics. Methods and Protocols’ is fresh in ideas and a useful manual for scientists, researchers, scholars, students and the wider readership interested in plant metabolism and metabolite molecules. … The purpose of this volume is to provide information on basic practical questions concerning experimental work with metabolomics. … The book is a mix between a scientific treatise and a laboratory manual. As such it is an excellent one.” (Tadeusz Aniszewski, Plant Science Bulletin, Vol. 58 (4), 2012)

Editors and Affiliations

  • , Dept. Computer Sciences, Aberystwyth University, Aberystwyth, United Kingdom

    Nigel W. Hardy

  • Plant Research International, Wageningen, Netherlands

    Robert D. Hall

Bibliographic Information

  • Book Title: Plant Metabolomics

  • Book Subtitle: Methods and Protocols

  • Editors: Nigel W. Hardy, Robert D. Hall

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-61779-594-7

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC 2012

  • Hardcover ISBN: 978-1-61779-593-0Published: 17 February 2012

  • Softcover ISBN: 978-1-4939-5748-4Published: 23 August 2016

  • eBook ISBN: 978-1-61779-594-7Published: 22 February 2012

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XIII, 340

  • Topics: Plant Sciences, Plant Physiology, Computer Appl. in Life Sciences

Buy it now

Buying options

eBook USD 89.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 119.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 169.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access