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Single-Cell Analysis

Methods and Protocols

  • Book
  • © 2012

Overview

Editors:
  1. Sara Lindström

    1. POOLIA Sweden, Stockholm, Sweden

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  2. Helene Andersson-Svahn

    1. , Division of Nanobiotechnology, Royal Institute of Technology, Stockholm, Sweden

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Provides clear guidelines for conducting difficult miniaturized techniques
  • Includes detailed and user-friendly protocols from the leading scientists in this field
  • Feature key tips and expert implementation advice to ensure successful results

Part of the book series: Methods in Molecular Biology (MIMB, volume 853)

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Table of contents (16 protocols)

  1. Front Matter

    Pages i-x
    Download chapter PDF

  2. Introduction: Why Analyze Single Cells?

    • Dino Di Carlo, Henry Tat Kwong Tse, Daniel R. Gossett
    Pages 1-10

  4. Miniaturized Solutions

    1. Front Matter

      Pages 39-39
      Download chapter PDF

    2. Single-Cell Culture in Microwells

      • Sara Lindström, Helene Andersson-Svahn
      Pages 41-52

    3. Isodielectric Separation and Analysis of Cells

      • Michael D. Vahey, Joel Voldman
      Pages 53-63

    4. Single Cell Electroporation Using Microfluidic Devices

      • Séverine Le Gac, Albert van den Berg
      Pages 65-82

    5. Perfusion Culture of Mammalian Cells in a Microfluidic Channel with a Built-In Pillar Array

      • Chi Zhang
      Pages 83-94

    6. Padlock Probes and Rolling Circle Amplification for Detection of Repeats and Single-Copy Genes in the Single-Cell Comet Assay

      • Sara Henriksson, Mats Nilsson
      Pages 95-103

    7. Droplet Microfluidics for Single-Cell Analysis

      • Eric Brouzes
      Pages 105-139

    8. Screening of Antigen-Specific Antibody-Secreting Cells

      • Hiroyuki Kishi, Aishun Jin, Tatsuhiko Ozawa, Kazuto Tajiri, Tsutomu Obata, Atsushi Muraguchi
      Pages 141-150

    9. Analysis of Single Eukaryotic Cells Using Raman Tweezers

      • Elsa Correia Faria, Peter Gardner
      Pages 151-167

    10. Single-Cell Microinjection Technologies

      • Yan Zhang
      Pages 169-176

    11. Ultrasonic Manipulation of Single Cells

      • Martin Wiklund, Björn Önfelt
      Pages 177-196

  5. Reviews on Chosen Subjects Within the Field

    1. Front Matter

      Pages 197-197
      Download chapter PDF

    2. Expanding the Horizons for Single-Cell Applications on Lab-on-a-Chip Devices

      • Soo Hyeon Kim, Dominique Fourmy, Teruo Fujii
      Pages 199-210

    3. Analytical Technologies for Integrated Single-Cell Analysis of Human Immune Responses

      • Ayça Yalçın, Yvonne J. Yamanaka, J. Christopher Love
      Pages 211-235
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Keywords

About this book

Today, cells are commonly analyzed en masse, with thousands of cells per sample, yielding results on the average response of the cells. However, cellular heterogeneity implies that in order to learn more about cellular behaviour, it is important to study how individual cells respond, one by one. In Single-Cell Analysis: Methods and Protocols, experts in the field provide an update on the field of single-cell analysis wherein the latest findings and applications are described in detail.  The methods described in this book include a few examples of conventional methods and several examples of miniaturized methods.  Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

 

Authoritative and accessible, Single-Cell Analysis: Methods and Protocols encourages readers to explore new ways of studying cells that may help lead to exciting new discoveries.

Editors and Affiliations

  • POOLIA Sweden, Stockholm, Sweden

    Sara Lindström

  • , Division of Nanobiotechnology, Royal Institute of Technology, Stockholm, Sweden

    Helene Andersson-Svahn

Bibliographic Information

  • Book Title: Single-Cell Analysis

  • Book Subtitle: Methods and Protocols

  • Editors: Sara Lindström, Helene Andersson-Svahn

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-61779-567-1

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC 2012

  • Hardcover ISBN: 978-1-61779-566-4Published: 10 February 2012

  • Softcover ISBN: 978-1-4939-5921-1Published: 23 August 2016

  • eBook ISBN: 978-1-61779-567-1Published: 10 February 2012

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: X, 238

  • Topics: Cell Biology, Cell Physiology

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