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  • © 2012

Leucocytes

Methods and Protocols

  • Provides detailed protocols and practical advice on a variety of modern approaches to the study of leucocytes
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 844)

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Table of contents (20 protocols)

  1. Front Matter

    Pages i-xi
  2. ENU-Based Phenotype-Driven Screening

    • Vera M. Ripoll, Philip L. Kong, Paul K. Potter
    Pages 1-13
  3. Detection and Quantification of Cytokines and Other Biomarkers

    • Evan L. Chiswick, Elizabeth Duffy, Brian Japp, Daniel Remick
    Pages 15-30
  4. Flow Cytometry Analysis of Cell Cycling and Proliferation in Mouse Hematopoietic Stem and Progenitor Cells

    • Valérie Barbier, Bianca Nowlan, Jean-Pierre Lévesque, Ingrid G. Winkler
    Pages 31-43
  5. Analyzing Cell Death Events in Cultured Leukocytes

    • Karin Christenson, Fredrik B. Thorén, Johan Bylund
    Pages 65-86
  6. Towards a Four-Dimensional View of Neutrophils

    • Ben A. Croker, Andrew W. Roberts, Nicos A. Nicola
    Pages 87-99
  7. Measurement of Oxidative Burst in Neutrophils

    • Yu Chen, Wolfgang G. Junger
    Pages 115-124
  8. Measurement of Neutrophil Elastase, Proteinase 3, and Cathepsin G Activities using Intramolecularly Quenched Fluorogenic Substrates

    • Brice Korkmaz, Sylvie Attucci, Christophe Epinette, Elodie Pitois, Marie-Lise Jourdan, Luiz Juliano et al.
    Pages 125-138
  9. The Macrophage

    • Chris P. Verschoor, Alicja Puchta, Dawn M. E. Bowdish
    Pages 139-156
  10. Measuring the Inflammasome

    • Olaf Groß
    Pages 199-222
  11. Arginine and Macrophage Activation

    • Mònica Comalada, Andree Yeramian, Manuel Modolell, Jorge Lloberas, Antonio Celada
    Pages 223-235
  12. Immunodetection of Granzyme B Tissue Distribution and Cellular Localisation

    • Catherina H. Bird, Corrine Hitchen, Mark Prescott, Ian Harper, Phillip I. Bird
    Pages 237-250
  13. Detection of Human and Mouse Granzyme B Activity in Cell Extracts

    • Sarah Elizabeth Stewart, Matthew Stephen James Mangan, Phillip Ian Bird, Dion Kaiserman
    Pages 251-260

About this book

The innate immune response is a crucial component of early resistance to infection, and it is now revealing increasing levels of complexity. The ability to modify the genome in vivo, has facilitated understanding of complex interactions between leucocytes and other components of the immune system, and phenotype-driven strategies using chemical mutagenesis have placed another powerful weapon in the armamentarium. In, Leucocytes: Methods and Protocols provides detailed protocols and practical advice on a variety of modern approaches to the study of leucocytes and their products.  Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Leucocytes: Methods and Protocols seeks to aid scientists in further study into the immune system and leucocytes.

Editors and Affiliations

  • School of Dentistry, University of Queensland, Brisbane, Australia

    Robert B. Ashman

Bibliographic Information

  • Book Title: Leucocytes

  • Book Subtitle: Methods and Protocols

  • Editors: Robert B. Ashman

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-61779-527-5

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC 2012

  • Hardcover ISBN: 978-1-61779-526-8Published: 21 January 2012

  • Softcover ISBN: 978-1-4939-6231-0Published: 23 August 2016

  • eBook ISBN: 978-1-61779-527-5Published: 21 January 2012

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 296

  • Topics: Cell Biology, Developmental Biology

Buy it now

Buying options

eBook USD 84.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 159.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 109.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access