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  • © 2012

Cardiovascular Development

Methods and Protocols

  • Covers state-of-the-art techniques in the field of cardiovascular development
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 843)

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Table of contents (28 protocols)

  1. Front Matter

    Pages i-xiv
  2. Model Organisms

    1. Front Matter

      Pages 1-1
  3. Model organisms

    1. Use of Whole Embryo Culture for Studying Heart Development

      • Calvin T. Hang, Ching-Pin Chang
      Pages 3-9
    2. Quantifying Cardiac Functions in Embryonic and Adult Zebrafish

      • Tiffany Hoage, Yonghe Ding, Xiaolei Xu
      Pages 11-20
    3. Morpholino Injection in Xenopus

      • Panna Tandon, Chris Showell, Kathleen Christine, Frank L. Conlon
      Pages 29-46
    4. Visualizing Vascular Networks in Zebrafish: An Introduction to Microangiography

      • Christopher E. Schmitt, Melinda B. Holland, Suk-Won Jin
      Pages 59-67
    5. Whole-Mount Confocal Microscopy for Vascular Branching Morphogenesis

      • Yoh-suke Mukouyama, Jennifer James, Joseph Nam, Yutaka Uchida
      Pages 69-78
    6. Miniaturized Assays of Angiogenesis In Vitro

      • May J. Reed, Robert B. Vernon
      Pages 87-98
  4. Cell and Molecular Biology Methods

    1. Front Matter

      Pages 99-99
  5. Cell and Molecular Biology Methods

    1. Quantification of Myocyte Chemotaxis: A Role for FAK in Regulating Directional Motility

      • Britni Zajac, Zeenat S. Hakim, Morgan V. Cameron, Oliver Smithies, Joan M. Taylor
      Pages 111-123
    2. Isolation and Characterization of Vascular Endothelial Cells from Murine Heart and Lung

      • Yixin Jin, Yang Liu, Marc Antonyak, Xu Peng
      Pages 147-154
    3. Vascular Smooth Muscle Cells: Isolation, Culture, and Characterization

      • Richard P. Metz, Jan L. Patterson, Emily Wilson
      Pages 169-176
    4. C-kit Expression Identifies Cardiac Precursor Cells in Neonatal Mice

      • Michael Craven, Michael I. Kotlikoff, Alyson S. Nadworny
      Pages 177-189

About this book

sCongenital heart disease is the leading cause of infant death and affects approximately one in every 100 babies born in the United States. The study of cardiovascular development has acquired new momentum in last twenty years due to the advancement of modern molecular biology and new available equipments and techniques. In Cardiovascular Development: Methods and Protocols expert researchers in the field in the field detail many of the methods which are now commonly used in the field of cardiovascular development research. These include methods and technique for using different organisms for cardiovascular developmental research, using cell and molecular biology methods to study cardiovascular development, as well as other available techniques for cardiovascular development research. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Cardiovascular Development: Methods and Protocols seeks to aid scientists in understanding new state-of-the-art techniques in the field of cardiovascular development research including in vivo imaging and Bioinformatics.

Reviews

From the reviews:

“This is an up-to-date review of current research techniques used in the field of cardiovascular development. … The book successfully instructs researchers on proper experimental techniques, giving step-by-step instructions, required for research in this field. … this book is intended for use by both novice and experienced basic science researchers. … This book is an excellent reference for any researcher in this area.” (Patrick T. Campbell, Doody’s Book Reviews, April, 2012)

Editors and Affiliations

  • College of Medicine, Dept. Systems Biology &, Texas A&M Health Science Center, Temple, USA

    Xu Peng

  • School of Veterinary Medicine, Department of Molecular Medicine, Cornell University, Ithaca, USA

    Marc Antonyak

Bibliographic Information

  • Book Title: Cardiovascular Development

  • Book Subtitle: Methods and Protocols

  • Editors: Xu Peng, Marc Antonyak

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-61779-523-7

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC 2012

  • Hardcover ISBN: 978-1-61779-522-0Published: 06 January 2012

  • Softcover ISBN: 978-1-4939-5853-5Published: 23 August 2016

  • eBook ISBN: 978-1-61779-523-7Published: 07 January 2012

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XIV, 341

  • Topics: Molecular Medicine, Cardiology

Buy it now

Buying options

eBook USD 84.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 159.00
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 109.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access