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  • Book
  • © 2012

Protein NMR Techniques

  • Comprehensive description of the latest innovations in the field of protein NMR
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 831)

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Table of contents (24 protocols)

  1. Front Matter

    Pages i-xiv
  2. A Novel Bacterial Expression Method with Optimized Parameters for Very High Yield Production of Triple-Labeled Proteins

    • Victoria Murray, Yuefei Huang, Jianglei Chen, Jianjun Wang, Qianqian Li
    Pages 1-18
  3. Isotopic Labeling of Heterologous Proteins in the Yeast Pichia pastoris and Kluyveromyces lactis

    • Toshihiko Sugiki, Osamu Ichikawa, Mayumi Miyazawa-Onami, Ichio Shimada, Hideo Takahashi
    Pages 19-36
  4. Isotope Labeling in Insect Cells

    • Krishna Saxena, Arpana Dutta, Judith Klein-Seetharaman, Harald Schwalbe
    Pages 37-54
  5. Isotope Labeling in Mammalian Cells

    • Arpana Dutta, Krishna Saxena, Harald Schwalbe, Judith Klein-Seetharaman
    Pages 55-69
  6. Cell-Free Protein Production for NMR Studies

    • Mitsuhiro Takeda, Masatsune Kainosho
    Pages 71-84
  7. Cell-Free Membrane Protein Expression for Solid-State NMR

    • Alaa Abdine, Kyu-Ho Park, Dror E. Warschawski
    Pages 85-109
  8. Expression and Purification of Src-family Kinases for Solution NMR Studies

    • Andrea Piserchio, David Cowburn, Ranajeet Ghose
    Pages 111-131
  9. NMR Studies of Large Protein Systems

    • Shiou-Ru Tzeng, Ming-Tao Pai, Charalampos G. Kalodimos
    Pages 133-140
  10. Bacterial Production and Solution NMR Studies of a Viral Membrane Ion Channel

    • Jolyon K. Claridge, Jason R. Schnell
    Pages 165-179
  11. Preparation of the Modular Multi-Domain Protein RPA for Study by NMR Spectroscopy

    • Chris A. Brosey, Marie-Eve Chagot, Walter J. Chazin
    Pages 181-195
  12. NMR Studies of Protein–RNA Interactions

    • Carla A. Theimer, Nakesha L. Smith, May Khanna
    Pages 197-218
  13. NMR Studies of Protein–Ligand Interactions

    • Michael Goldflam, Teresa Tarragó, Margarida Gairí, Ernest Giralt
    Pages 233-259
  14. In-Cell NMR Spectroscopy in Escherichia coli

    • Kirsten E. Robinson, Patrick N. Reardon, Leonard D. Spicer
    Pages 261-277
  15. Solid-State NMR Spectroscopy of Protein Complexes

    • Shangjin Sun, Yun Han, Sivakumar Paramasivam, Si Yan, Amanda E. Siglin, John C. Williams et al.
    Pages 303-331

About this book

The field of protein NMR spectroscopy has rapidly expanded into new areas of biochemistry, molecular biology and cell biology research that were impossible to study as recently as ten years ago. This third edition of Protein NMR Techniques, expands upon the previous editions with current, detailed authoritative but down-to-earth descriptions of new methodologies. These include techniques for NMR sample preparation, solution and solid state NMR methodologies and data processing. Written in the highly successful Methods in Molecular Biologyâ„¢ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

 

Authoritative and practical, Protein NMR Techniques,Third Edition, seeks to aid scientists in understanding the latest innovations in the field of protein NMR.

Editors and Affiliations

  • University of Albany, State University of New York, Albany, USA

    Alexander Shekhtman, David S. Burz

Bibliographic Information

  • Book Title: Protein NMR Techniques

  • Editors: Alexander Shekhtman, David S. Burz

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-61779-480-3

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC 2012

  • Hardcover ISBN: 978-1-61779-479-7Published: 14 December 2011

  • Softcover ISBN: 978-1-4939-6195-5Published: 23 August 2016

  • eBook ISBN: 978-1-61779-480-3Published: 15 December 2011

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 3

  • Number of Pages: XIV, 518

  • Topics: Protein Science, Spectroscopy/Spectrometry

Buy it now

Buying options

eBook USD 89.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 119.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 169.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access