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  • © 2012

DNA Electrophoresis Protocols for Forensic Genetics

Editors:

  • Offers comprehensive coverage of the most modern current electrophoretic protocols and interpretation guidelines used in forensic genetics
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 830)

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Table of contents (26 protocols)

  1. Front Matter

    Pages i-xiii
  2. Current Protocols on Capillary Electrophoresis of Amplified DNA Fragments

    1. Front Matter

      Pages 1-1
  3. Current Protocols on Capillary Electrophoresis of amplified DNA fragments

    1. An Overview of DNA Typing Methods for Human Identification: Past, Present, and Future

      • Robyn Thompson, Silvia Zoppis, Bruce McCord
      Pages 3-16
    2. Capillary Electrophoresis of an X-Chromosome STR Decaplex for Kinship Deficiency Cases

      • Leonor Gusmão, Cíntia Alves, Iva Gomes, Paula Sánchez-Diz
      Pages 57-71
    3. A 34-plex Autosomal SNP Single Base Extension Assay for Ancestry Investigations

      • C. Phillips, M. Fondevila, Maria Victoria Lareau
      Pages 109-126
    4. DNA Extraction from Aged Skeletal Samples for STR Typing by Capillary Electrophoresis

      • René Huel, Sylvain Amory, Ana Bilić, Stojko Vidović, Edin Jasaragić, Thomas J. Parsons
      Pages 185-198

About this book

Forensic DNA profiling procedures are mainly based on high resolution and high throughput capillary electrophoresis separation and detection systems of  PCR amplicons obtained from DNA genomic markers with different  inheritance patterns. In DNA Electrophoresis Protocols for Forensic Genetics, expert researchers in the field detail many of the protocols and methods which are now commonly used to perform forensic DNA profiling. It  includes protocols for  profiling of autosomal STRs, Y-STRs, X-STRs, autosomal SNPs, INDELS, Y-SNPs, mtDNA-SNPs, and  mtDNA  hypervariable regions HV1 and HV2 . Protocols for molecular identification of non-human species and mRNA profiling for body fluid identification are also included. Written in the highly successful  Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

Reviews

From the reviews:

“The purpose is to offer a comprehensive book on the most modern current electrophoretic protocols and interpretation guidelines used in forensic genetics for the analysis of amplified human DNA fragments and DNA sequencing. … researchers, forensic experts, and others interested in the field of DNA electrophoresis protocols will find this book of immense help. … This is a unique resource that students, researchers, and forensic experts will find both interesting and helpful.” (Omer Iqbal, Doody’s Book Reviews, May, 2012)

Editors and Affiliations

  • Ciencias Forenses, Servicio de Biología, Instituto Nacional de Toxicología y, Las Rozas, Spain

    Antonio Alonso

Bibliographic Information

  • Book Title: DNA Electrophoresis Protocols for Forensic Genetics

  • Editors: Antonio Alonso

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-61779-461-2

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC 2012

  • Hardcover ISBN: 978-1-61779-460-5Published: 03 December 2011

  • Softcover ISBN: 978-1-4939-6219-8Published: 23 August 2016

  • eBook ISBN: 978-1-61779-461-2Published: 04 December 2011

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XIII, 394

  • Topics: Human Genetics, Electrophoresis

Buy it now

Buying options

eBook USD 84.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 159.00
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 109.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access