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Recombinant Gene Expression

  • Book
  • © 2012

Overview

Editors:
  1. Argelia Lorence

    1. Dept. Chemistry & Physics, Arkansas State University, Jonesboro, State University, USA

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  • Contains new protocols and topics not yet covered
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 824)

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Table of contents (35 protocols)

  1. Front Matter

    Pages i-xvi
    Download chapter PDF

  2. General Aspects

    1. Front Matter

      Pages 1-1
      Download chapter PDF

    2. Using Folding Promoting Agents in Recombinant Protein Production: A Review

      • Beatrix Fahnert
      Pages 3-36

    3. Routine Identity Confirmation of Recombinant Proteins by MALDI-TOF Mass Spectrometry

      • Brett J. Savary, Prasanna Vasu
      Pages 37-50

    4. A Matter of Packaging: Influence of Nucleosome Positioning on Heterologous Gene Expression

      • María de la Cruz Muñoz-Centeno, Gonzalo Millán-Zambrano, Sebastián Chávez
      Pages 51-64

    5. Tools of the Trade: Developing Antibody-Based Detection Capabilities for Recombinant Proteins

      • Maureen C. Dolan, Giuliana Medrano, Anthony McMickle, Carole L. Cramer
      Pages 65-105

  3. Prokaryotes

    1. Front Matter

      Pages 107-107
      Download chapter PDF

    2. Heat-Shock Protein Fusion Vectors for Improved Expression of Soluble Recombinant Proteins in Escherichia coli

      • Christos A. Kyratsous, Christos A. Panagiotidis
      Pages 109-129

    3. The Use of a Flagellar Export Signal for the Secretion of Recombinant Proteins in Salmonella

      • Ferenc Vonderviszt, Ráchel Sajó, József Dobó, Péter Závodszky
      Pages 131-143

    4. Optimization of Purification Protocols Based on the Step-by-Step Monitoring of the Protein Aggregates in Soluble Fractions

      • Ario de Marco
      Pages 145-154

    5. Heterologous Protein Expression by Lactococcus lactis

      • Julio Villatoro-Hernández, Oscar P. Kuipers, Odila Saucedo-Cárdenas, Roberto Montes-de-Oca-Luna
      Pages 155-165

    6. An Extended Suite of Genetic Tools for Use in Bacteria of the Halomonadaceae: An Overview

      • Montserrat Argandoña, Carmen Vargas, Mercedes Reina-Bueno, Javier Rodríguez-Moya, Manuel Salvador, Joaquín J. Nieto
      Pages 167-201

    7. Regulated Recombinant Protein Production in the Antarctic Bacterium Pseudoalteromonas haloplanktis TAC125

      • Valentina Rippa, Rosanna Papa, Maria Giuliani, Cinzia Pezzella, Ermenegilda Parrilli, Maria Luisa Tutino et al.
      Pages 203-218

    8. A Novel Strategy for the Construction of Genomic Mutants of the Antarctic Bacterium Pseudoalteromonas haloplanktis TAC125

      • Maria Giuliani, Ermenegilda Parrilli, Cinzia Pezzella, Valentina Rippa, Angela Duilio, Gennaro Marino et al.
      Pages 219-233

    9. A New Bacterial Co-expression System for Over-expressing Soluble Protein and Validating Protein–Protein Interaction

      • Jumei Zeng, Zheng-Guo He
      Pages 235-249

    10. Heterologous High-Level Gene Expression in the Photosynthetic Bacterium Rhodobacter capsulatus

      • Nadine Katzke, René Bergmann, Karl-Erich Jaeger, Thomas Drepper
      Pages 251-269

    11. Plasmid DNA Production for Therapeutic Applications

      • Alvaro R. Lara, Octavio T. Ramírez
      Pages 271-303
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Keywords

About this book

Studies related to recombinant gene expression have brought new advance such as  the emergence of the “omics” technologies. While Escherichia coli, Sacharomyces cerevisiae and insect cells continue to be the dominant production platforms of recombinant proteins. In Recombinant Gene Expression: Review and Protocols, Third Edition, expert researchers in the field detail many of the methods now commonly used to study recombinant gene expression. These include methods and techniques for bacteria, lower eukaryotes, fungi, plants and plant cells, and animals and animal cells. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

 

Authoritative and practical, Recombinant Gene Expression: Review and Protocols, Third Edition seeks to aid scientists in the further study of this crucially important research into recombinant gene expression.

Editors and Affiliations

  • Dept. Chemistry & Physics, Arkansas State University, Jonesboro, State University, USA

    Argelia Lorence

Bibliographic Information

  • Book Title: Recombinant Gene Expression

  • Editors: Argelia Lorence

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-61779-433-9

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC 2012

  • Hardcover ISBN: 978-1-61779-432-2Published: 13 December 2011

  • Softcover ISBN: 978-1-4939-6221-1Published: 23 August 2016

  • eBook ISBN: 978-1-61779-433-9Published: 14 December 2011

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 3

  • Number of Pages: XVI, 649

  • Number of Illustrations: 105 b/w illustrations

  • Topics: Human Genetics, Gene Expression

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