Editors:
- Features a well-balanced collection of established methods and cutting-edge techniques
- Includes easy to follow, step-by-step protocols, provided in clear detail
- Points out expert tips and implementation advice in order to ensure successful results
- Includes supplementary material: sn.pub/extras
Part of the book series: Methods in Molecular Biology (MIMB, volume 805)
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Table of contents (23 protocols)
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Front Matter
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Review
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Front Matter
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Translation Extract Preparation
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Front Matter
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Basic Ribosome Display and Related Selection Methods
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Front Matter
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Applications of Ribosome Display Methods Using Natural Amino Acids
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Front Matter
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About this book
Display technologies have become a very powerful way of generating therapeutic lead molecules and specific reagents for increasing our understanding of biology; however, despite being first described shortly after phage display, the use of ribosome display and related methods have been much less widespread. Since this is in part due to the complexity of the methods, Ribosome Display and Related Technologies: Methods and Protocols seeks to extend their use by collecting expert contributions describing these detailed protocols. The protocols described range from well-established methods that have been used for a decade to generate high affinity antibodies, which are already in the clinic, to methods that are in their early stages of application such as display of peptides incorporating non-canonical amino acids. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
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Invaluable and easy to use, Ribosome Display and Related Technologies: Methods and Protocols will be of great benefit to those with general molecular biology or protein engineering experience who wish to select peptides or proteins by display, those with phage display experience who would benefit from the application of ribosome display, as well as those with some ribosome display experience who would like to expand the range of applications to which they are applying the technology.Editors and Affiliations
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, Antibody Discovery & Protein Engineering, MedImmune Limited, Cambridge, United Kingdom
Julie A. Douthwaite, Ronald H. Jackson
Bibliographic Information
Book Title: Ribosome Display and Related Technologies
Book Subtitle: Methods and Protocols
Editors: Julie A. Douthwaite, Ronald H. Jackson
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-61779-379-0
Publisher: Humana Totowa, NJ
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media, LLC 2012
Hardcover ISBN: 978-1-61779-378-3Published: 17 November 2011
Softcover ISBN: 978-1-4939-5872-6Published: 23 August 2016
eBook ISBN: 978-1-61779-379-0Published: 19 November 2011
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: XIII, 424
Topics: Protein Science