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  • © 2012

Carbohydrate Microarrays

Methods and Protocols

Editors:

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 808)

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Table of contents (28 protocols)

  1. Front Matter

    Pages i-xv
  2. Recent Advances and Future Challenges in Glycan Microarray Technology

    • José L. de Paz, Peter H. Seeberger
    Pages 1-12
  3. General Consideration on Sialic Acid Chemistry

    • Hongzhi Cao, Xi Chen
    Pages 31-56
  4. Synthesis of Azido-Functionalized Carbohydrates for the Design of Glycoconjugates

    • Samy Cecioni, David Goyard, Jean-Pierre Praly, Sébastien Vidal
    Pages 57-68
  5. Neoglycolipid-Based Oligosaccharide Microarray System: Preparation of NGLs and Their Noncovalent Immobilization on Nitrocellulose-Coated Glass Slides for Microarray Analyses

    • Yan Liu, Robert A. Childs, Angelina S. Palma, Maria A. Campanero-Rhodes, Mark S. Stoll, Wengang Chai et al.
    Pages 117-136
  6. Production of Fluorous-Based Microarrays with Uncharged Carbohydrates

    • Sahana K. Nagappayya, Nicola L. B. Pohl
    Pages 149-153
  7. Immobilization of Polyacrylamide-Based Glycoconjugates on Solid Phase in Immunosorbent Assays

    • Oxana E. Galanina, Alexander A. Chinarev, Nadezhda V. Shilova, Marina A. Sablina, Nicolai V. Bovin
    Pages 167-182
  8. Glycoarray by DNA-Directed Immobilization

    • François Morvan, Yann Chevolot, Jing Zhang, Albert Meyer, Sébastien Vidal, Jean-Pierre Praly et al.
    Pages 195-219
  9. Fabrication of Carbohydrate Surfaces by Using Non-derivatised Oligosaccharides

    • Jonathan Popplewell, Marcus Swann, Gavin Brown, Bob Lauder
    Pages 221-229
  10. Polysaccharide Microarrays: Application to the Identification of Heparan Sulphate Mimetics

    • Julien Dheur, Nabil Dendane, Rémi Desmet, Fatima Dahmani, Gauthier Goormachtigh, Jérome Vicogne et al.
    Pages 231-240
  11. Carbohydrate Antigen Microarrays

    • Denong Wang
    Pages 241-249
  12. Probing Virus–Glycan Interactions Using Glycan Microarrays

    • Jamie Heimburg-Molinaro, Mary Tappert, Xuezheng Song, Yi Lasanajak, Gillian Air, David F. Smith et al.
    Pages 251-267
  13. MALDI-ToF MS Analysis of Glycosyltransferase Activities on Gold Surface Arrays

    • Nicolas Laurent, Rose Haddoub, Josef Voglmeir, Sabine L. Flitsch
    Pages 269-284

About this book

Carbohydrate microarrays emerged as a key technology for the deciphering of the glycospace by providing a multiplex technology where tens to hundreds of carbohydrates/protein interactions can be probed in parallel. Carbohydrate Microarrays: Methods and Protocols aims to give the reader the theoretical and experimental clues necessary for the fabrication and implementation of carbohydrate microarrays. This requires three essential steps: 1) to obtain the carbohydrate probes (monosacharides, oligosacchrides, polysacchairdes, glycoconjugates or glycoclusters), 2) to immobilize these probes, and 3) to implement the protocols for biological/biochemical interaction with the desired target. This volume gives an overview of carbohydrate microarray and carbohydrate chemistry and illustrates different detection techniques and their applications. Written in the successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.

 

Authoritative and easily accessible, Carbohydrate Microarrays: Methods and Protocols compiles a catalogue of protocols on carbohydrate microarrays to span the needs of researchers around the globe. 

Editors and Affiliations

  • CNRS UMR 5270, Inst. Nanotechnologies de Lyon, Université Lyon 1, Ecully, France

    Yann Chevolot

Bibliographic Information

  • Book Title: Carbohydrate Microarrays

  • Book Subtitle: Methods and Protocols

  • Editors: Yann Chevolot

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-61779-373-8

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC 2012

  • Hardcover ISBN: 978-1-61779-372-1Published: 05 November 2011

  • Softcover ISBN: 978-1-4939-5866-5Published: 23 August 2016

  • eBook ISBN: 978-1-61779-373-8Published: 06 November 2011

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XV, 427

  • Number of Illustrations: 109 b/w illustrations, 28 illustrations in colour

  • Topics: Protein Science, Microarrays

Buy it now

Buying options

eBook USD 99.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 129.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 169.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access