Editors:
- Supplies techniques that can be adapted to obtain cell cultures from diseased cells to gain understanding of the pathogenic mechanisms involved
- Features cutting-edge methods ready for the laboratory
- Includes expert tips and implementation advice to ensure successful results
Part of the book series: Methods in Molecular Biology (MIMB, volume 806)
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Table of contents (27 protocols)
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Front Matter
About this book
Human cell culture is not a new topic, but the development of new molecular techniques and reagents which can be used to investigate cell function and the responsible intracellular mechanisms make it a continuing requirement. This third edition of Human Cell Culture Protocols expands upon the previous editions with current, detailed protocols for the isolation and culture of a range of primary cells from human tissues. With new chapters on pancreatic cells needed for basic studies on the pathogenesis of diabetes and for their application for islet transplantation, the book also delves into protocols for hepatocytes, skin cells, lung cells, parathyroid cells, gastric cells, renal cells, adipocytes, ovarian cells, bone cells, vascular smooth muscle cells, vascular endothelial cells, regulatory T cells, blood mononuclear cells, as well as new techniques being applied to human cell culture, particularly the use of biocompatible scaffolds to grow cells, the in vitro use of laser microdissection to isolate cells from culture, and automated cell culture. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Human Cell Culture Protocols, Third Edition makes it possible for a worker with basic cell culture training, whether in the fields of cell biology, gene therapy, and cell transplantation, to prepare cell cultures of the specific cell type necessary to forward their vital research.
Editors and Affiliations
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Institute of Liver Studies, King’s College Hospital, London, United Kingdom
Ragai R. Mitry
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School of Medicine, Inst. Liver Studies, King's College London, London, United Kingdom
Robin D. Hughes
Bibliographic Information
Book Title: Human Cell Culture Protocols
Editors: Ragai R. Mitry, Robin D. Hughes
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-61779-367-7
Publisher: Humana Totowa, NJ
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media, LLC 2012
Hardcover ISBN: 978-1-61779-366-0Published: 05 November 2011
Softcover ISBN: 978-1-4939-6208-2Published: 23 August 2016
eBook ISBN: 978-1-61779-367-7Published: 06 November 2011
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 3
Number of Pages: XIV, 435
Topics: Cell Biology, Cell Culture